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Proteins neighboring 18S rRNA conserved sequences 609–618 and 1047–1061 within the 40S human ribosomal subunit

  • ALEXEY A. MALYGIN (a1), MIKHAIL I. DOBRIKOV (a1), MARINA N. REPKOVA (a1), GENNADI V. SHISHKIN (a1), ALIYA G. VEN'YAMINOVA (a1) and GALINA G. KARPOVA (a1)...

Abstract

The structure of human 40S ribosomal subunits has been probed by a cross-linking strategy based on the use of oligonucleotide derivatives that modify proteins in the vicinity of specific 18S rRNA sequences. The oligonucleotide derivatives carried a p-azidoperfluorobenzamide group at the 5′ ends and were complementary to 18S rRNA sequences 609–618 and 1047–1061, homologous to the highly conserved regions designated as the “530 stem-loop” and “790 stem-loop”, respectively, in Escherichia coli 16S rRNA. Ribosomal proteins surrounding these sequences were the main targets of the cross-linking. Proteins S3 and S5 were cross-linked to the derivative complementary to the sequence 609–618, and proteins S2 and S3 were modified by the derivative complementary to the sequence 1047–1061. Cross-linking was not affected by binding of 40S subunits to either poly(U) or poly(U) and Phe-tRNAPhe.

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Corresponding author

Reprint requests to: Galina G. Karpova, Novosibirsk Institute of Bioorganic Chemistry, Siberian Branch of the Russian Academy of Sciences, pr. Lavrentjeva 8, Novosibirsk, 630090, Russia; e-mail: karpova@niboch.nsc.ru.

Keywords

Proteins neighboring 18S rRNA conserved sequences 609–618 and 1047–1061 within the 40S human ribosomal subunit

  • ALEXEY A. MALYGIN (a1), MIKHAIL I. DOBRIKOV (a1), MARINA N. REPKOVA (a1), GENNADI V. SHISHKIN (a1), ALIYA G. VEN'YAMINOVA (a1) and GALINA G. KARPOVA (a1)...

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