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An engineered class I transfer RNA with a class II tertiary fold

  • T. AMAR NISSAN (a1), BRYANT OLIPHANT (a1) and JOHN J. PERONA (a1)

Abstract

Structure-based engineering of the tertiary fold of Escherichia coli tRNA2Gln has enabled conversion of this transfer RNA to a class II structure while retaining recognition properties of a class I glutamine tRNA. The new tRNA possesses the 20-nt variable stem-loop of Thermus thermophilus tRNASer. Enlargement of the D-loop appears essential to maintaining a stable tertiary structure in this species, while rearrangement of a base triple in the augmented D-stem is critical for efficient glutaminylation. These data provide new insight into structural determinants distinguishing the class I and class II tRNA folds, and demonstrate a marked sensitivity of glutaminyl-tRNA synthetase to alteration of tRNA tertiary structure.

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Corresponding author

Reprint requests to: John J. Perona, Department of Chemistry, University of California at Santa Barbara, Santa Barbara, California 93106-9510, USA; email: perona@chem.ucsb.edu.

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An engineered class I transfer RNA with a class II tertiary fold

  • T. AMAR NISSAN (a1), BRYANT OLIPHANT (a1) and JOHN J. PERONA (a1)

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