Skip to main content Accessibility help
×
Home

Comparative study of methanogens in one- and two-stage anaerobic digester treating food waste

  • Marta Kinnunen (a1), Daniel Hilderbrandt (a1), Stefan Grimberg (a1), Shane Rogers (a1) and Sumona Mondal (a2)...

Abstract

Changes in methanogenic archaea were investigated in pilot-scale experiments during one- and two-stage mesophilic anaerobic digestion (AD) of food waste. Methane yields were 379.7±75.3 liters of methane per kg of volatile solids [L-CH4 (kg-VS)−1] added to the system, during one-stage operation, and 446±922 L-CH4 (kg-VS)−1 added during two-stage operation. Populations of methanogenic archaea were monitored quantitatively by targeting the functional gene for methyl-coenzyme-M reductase (mcrA) using real-time quantitative polymerase chain reaction techniques. During one-stage operation, mean mcrA gene concentrations were 2.48×109±2.7×109 copies ml−1. Two-stage operation yielded mean mcrA gene concentrations of 9.85×108±8.2×108 copies ml−1 in the fermentation and 1.76×1010±8.5×109 copies ml−1 in the methanogenesis reactors, respectively. Diversity of archaea in the methanogenic reactors was investigated by denaturing gradient gel electrophoresis targeting the V3 region of 16S rRNA of archaea. The Shannon index (H) was 2.98 for one-stage operation and 7.29 for two-stage operation, suggesting greater archaeal diversity in the two-stage AD. The fivefold increase in methanogenic archaea populations during the two-stage operation, as indicated by mcrA gene concentration, corresponded to an increase in methane production rates. While the diversity may also be related to the stability of the microbial bioprocesses and improved methane production rates, the correlation between diversity and production rates should be studied further.

Copyright

Corresponding author

* Corresponding author: maki@env.dtu.dk

Footnotes

Hide All

Present address: Department of Environmental Engineering, Technical University of Denmark, Bygningstorvet, Building 115, Room 139, 2800 Kgs. Lyngby, Denmark.

Present address: San Jacinto River Authority, 1577 Dam Site Road, Conroe, Texas 77304, USA.

Footnotes

References

Hide All
1 Cho, J.K., Park, S.C., and Chang, H.N. 1995. Biochemical methane potential and solid state anaerobic digestion of Korean food wastes. Bioresource Technology 52:245253.
2 Dinsdale, R.M., Premier, G.C., Hawkes, F.R., and Hawkes, D.L. 2000. Two-stage anaerobic co-digestion of waste activated sludge and fruit/vegetable waste using inclined tubular digesters. Bioresource Technology 72:159168.
3 Klocke, M., Nettmann, E., Bergmann, I., Mundt, K., Souidi, K., Mumme, J., and Linke, B. 2008. Characterization of the methanogenic archaea within two-phase biogas reactor systems operated with plant biomass. Systematic and Applied Microbiology 31:190205.
4 Shin, S.G., Han, G., Lim, J., Lee, C., and Hwang, S. 2010. A comprehensive microbial insight into two-stage anaerobic digestion of food waste-recycling wastewater. Water Research 44:48384849.
5 Li, Y., Park, S.Y., and Zhu, J. 2011. Solid-state anaerobic digestion for methane production from organic waste. Renewable and Sustainable Energy Reviews 15:821826.
6 Shen, F., Yuan, H., Pang, Y., Chen, S., Zhu, B., Zou, D., Liu, Y., Ma, J., Yu, L., and Li, X. 2013. Performances of anaerobic co-digestion of fruit & vegetable waste (FVW) and food waste (FW): Single-phase vs. two-phase. Bioresource Technology 144:8085.
7 Angenent, L.T., Sung, S., and Raskin, L. 2002. Methanogenic population dynamics during startup of a full-scale anaerobic sequencing batch reactor treating swine waste. Water Research 36:46484654.
8 Liu, W.-T., Chan, O.-C., and Fang, H.H.P. 2002. Microbial community dynamics during start-up of acidogenic anaerobic reactors. Water Research 36:32033210.
9 Cho, K., Lee, J., Kim, W., and Hwang, S. 2013. Behavior of methanogens during start-up of farm-scale anaerobic digester treating swine wastewater. Process Biochemistry 48:14411445.
10 Ike, M., Inoue, D., Miyano, T., Liu, T.T., Sei, K., Soda, S., and Kadoshin, S. 2010. Microbial population dynamics during startup of a full-scale anaerobic digester treating industrial food waste in Kyoto eco-energy project. Bioresource Technology 101:39523957.
11 Eaton, A.D., Clesceri, L.S., Rice, E.W., Greenberg, A E., and Franson, M.A.H. 2005. APHA: Standard Methods for the Examination of Water and Wastewater. Centennial Edition, APHA, AWWA, WEF, Washington, DC.
12 Grimberg, S.J., Hilderbrandt, D., Kinnunen, M., and Rogers, S.W. (2014). Anaerobic digestion of food waste through the operation of a mesophilic two-phase pilot scale digester – assessment of variable loadings on system performance. Bioresource Technology. doi 10.1016/j.biortech.2014.09.001.
13 Metcalf, L., Eddy, H.P., and Tchobanoglous, G. 2004. Wastewater Engineering: Treatment and Reuse. McGraw-Hill, New York.
14 Rogers, S.W., Donnelly, M., Peed, L., Kelty, C.A., Mondal, S., Zhong, Z., and Shanks, O.C. 2011. Decay of bacterial pathogens, fecal indicators, and real-time quantitative PCR genetic markers in manure-amended soils. Applied and Environmental Microbiology 77:48394848.
15 Nettmann, E., Bergmann, I., Mundt, K., Linke, B., and Klocke, M. 2008. Archaea diversity within a commercial biogas plant utilizing herbal biomass determined by 16S rDNA and mcrA analysis. Journal of Applied Microbiology 105:18351850.
16 Luton, P.E., Wayne, J.M., Sharp, R.J., and Riley, P.W. 2002. The mcrA gene as an alternative to 16S rRNA in the phylogenetic analysis of methanogen populations in landfill. Microbiology 148:35213530.
17 Freitag, T.E. and Prosser, J.I. 2009. Correlation of methane production and functional gene transcriptional activity in a peat soil. Applied and Environmental Microbiology 75:66796687.
18 Takai, K. and Horikoshi, K. 2000. Rapid detection and quantification of members of the archaeal community by quantitative PCR using fluorogenic probes. Applied Environmental Microbiology 66:50665072.
19 Ovreås, L., Forney, L., Daae, F.L., and Torsvik, V. 1997. Distribution of bacterioplankton in meromictic Lake Saelenvannet, as determined by denaturing gradient gel electrophoresis of PCR-amplified gene fragments coding for 16S rRNA. Applied Environmental Microbiology 63:33673373.
20 Shannon, C.E. and Weaver, W. 1963. A Mathematical Theory of Communication. University of Illinois Press. Available at Web site http://dl.acm.org/citation.cfm?id=584093 (accessed September 13, 2014).
21 Bialek, K., Kim, J., Lee, C., Collins, G., Mahony, T., and O'Flaherty, V. 2011. Quantitative and qualitative analyses of methanogenic community development in high-rate anaerobic bioreactors. Water Research 45:12981308.
22 Lin, J., Zuo, J., Ji, R., Chen, X., Liu, F., Wang, K., and Yang, Y. 2012. Methanogenic community dynamics in anaerobic co-digestion of fruit and vegetable waste and food waste. Journal of Environmental Sciences 24:12881294.
23 Rincón, B., Borja, R., Martín, M.A., and Martín, A. 2010. Kinetic study of the methanogenic step of a two-stage anaerobic digestion process treating olive mill solid residue. Chemical Engineering Journal 160:215219.
24 Lv, W., Zhang, W., and Yu, Z. 2013. Evaluation of system performance and microbial communities of a temperature-phased anaerobic digestion system treating dairy manure: Thermophilic digester operated at acidic pH. Bioresource Technology 142:625632.
25 Traversi, D., Villa, S., Lorenzi, E., Degan, R., and Gilli, G. 2012. Application of a real-time qPCR method to measure the methanogen concentration during anaerobic digestion as an indicator of biogas production capacity. Journal of Environmental Management 111:173177.
26 Singh, A., Singh, R.S., Upadhyay, S.N., Joshi, C.G., Tripathi, A.K., and Dubey, S.K. 2012. Community structure of methanogenic archaea and methane production associated with compost-treated tropical rice-field soil. FEMS Microbiology Ecology 82:118134.
27 Lee, D.H., Behera, S.K., Kim, J.W., and Park, H.-S. 2009. Methane production potential of leachate generated from Korean food waste recycling facilities: A lab-scale study. Waste Management 29:876882.
28 Williams, J., Williams, H., Dinsdale, R., Guwy, A., and Esteves, S. 2013. Monitoring methanogenic population dynamics in a full-scale anaerobic digester to facilitate operational management. Bioresource Technology 140:234242.
29 Boon, N., Windt, W., Verstraete, W., and Top, E.M. 2002. Evaluation of nested PCR–DGGE (denaturing gradient gel electrophoresis) with group-specific 16S rRNA primers for the analysis of bacterial communities from different wastewater treatment plants. FEMS Microbiology Ecology 39:101112.
30 Kundu, K., Sharma, S., and Sreekrishnan, T.R. 2012. Effect of operating temperatures on the microbial community profiles in a high cell density hybrid anaerobic bioreactor. Bioresource Technology 118:502511.
31 Peng, J., Song, Y., Wang, Y., Yuan, P., and Liu, R. 2013. Spatial succession and metabolic properties of functional microbial communities in an anaerobic baffled reactor. International Biodeterioration and Biodegradation 80:6065.
32 Lim, J.W., Chen, C.-L., Ho, I.J.R., and Wang, J.-Y. 2013. Study of microbial community and biodegradation efficiency for single- and two-phase anaerobic co-digestion of brown water and food waste. Bioresource Technology 147:193201.

Keywords

Comparative study of methanogens in one- and two-stage anaerobic digester treating food waste

  • Marta Kinnunen (a1), Daniel Hilderbrandt (a1), Stefan Grimberg (a1), Shane Rogers (a1) and Sumona Mondal (a2)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed