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A Semi-Automated Bone Pretreatment System and the Pretreatment of Older and Contaminated Samples

Published online by Cambridge University Press:  18 July 2016

I A Law  and
R E M Hedges
I A Law
Affiliation:
Oxford Radiocarbon Accelerator Unit, Research Laboratory for Archaeology and the History of Art, University of Oxford, 6, Keble Road, Oxford OX1 3 QJ, United Kingdom
R E M Hedges
Affiliation:
Oxford Radiocarbon Accelerator Unit, Research Laboratory for Archaeology and the History of Art, University of Oxford, 6, Keble Road, Oxford OX1 3 QJ, United Kingdom
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Abstract

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A semi-automated continuous-flow system used to process archaeological bone to purified gelatin or amino acids for 14C dating is described. Powdered bone is retained in flow cells specifically designed to permit the sequential leaching of the bone with acid, alkali and water. Crude collagen obtained by this process is gelatinized, and than either purified directly using a macroporous cation exchange resin (BioRad AGMP-50), or hydrolyzed and the amino acids desalted on BioRad 50W-X8 resin. When compared with previous methods used by the laboratory, the new method allows more samples to be treated to a higher degree of purification. Examples of dates obtained on “standard” bones are presented, and confirm that no contamination is introduced from the components used in the new process.

Type
I. Sample Preparation and Measurement Techniques
Information
Radiocarbon , Volume 31 , Issue 3: June 20-25, 1988 Dubrovnik, Yugoslavia , 1989 , pp. 247 - 253
Copyright
Copyright © The American Journal of Science 

References

Aiken, G R, McKnight, D M, Wershaw, R L and MacCarthy, P, 1985, Humic substances in soil, sediment, and water, in Geochemistry, isolation and characterization: New York, John Wiley & Sons.Google Scholar
Gillespie, R, Hedges, R E M and Humm, M J, 1986, Routine AMS dating of bone and shell proteins, in Stuiver, M and Kra, R S, eds, 12th internatl 14C conf, Proc: Radiocarbon, v 28, no. 2A, p 451–456.Google Scholar
Hedges, R E M, Housley, R A, Law, I A, Perry, C and Gowlett, J A J, 1987, Datelist 6: Archaeometry, v 29, no. 2, p 289–306.Google Scholar
Hunt, S, 1985, Degradation of amino acids accompanying in vitro protein hydrolysis, in Barrett, G C, ed, Chemistry and biochemistry of the amino acids: London, Chapman and Hall, p 376–398.Google Scholar
Longin, R, 1971, New method of collagen extraction for radiocarbon dating: Nature, v 230, p 241–242.CrossRefGoogle Scholar
Protsch, R, 1975, The absolute dating of Upper Pleistocene Sub-Saharan fossil hominids and their place in human evolution: Jour Human Evolution, v 4, p 297–322.CrossRefGoogle Scholar
Stafford, T W Jr, Duhamel, R C, Haynes, C and Brendel, K, 1982, Isolation of proline and hydroxyproline from fossil bone: Life Science, v 31, p 931–938.CrossRefGoogle Scholar
Stafford, T W Jr, Jull, A J T, Brendel, K, Duhamel, R C, and Donahue, D, 1987, Study of bone radiocarbon dating accuracy at the University of Arizona NSF Accelerator Facility for Radioisotope Analysis: Radiocarbon, v 29, no. 1, p 24–44.Google Scholar