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Magic angle spinning NMR spectroscopy of membrane proteins

Published online by Cambridge University Press:  17 March 2009

Steven O. Smith ,
Kathryn. Aschheim  and
Michel Groesbeek
Steven O. Smith
Affiliation:
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520–8114
Kathryn. Aschheim
Affiliation:
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520–8114
Michel Groesbeek
Affiliation:
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520–8114
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Extract

The passage of molecules and information across cell membranes is mediated largely by membrane-spanning proteins acting as channels, pumps, receptors and enzymes. These proteins perform many tasks: they control electrochemical gradients across the membrane, receive signals from the environment or from other cells, convert light energy into chemical signals, transport small molecules into and out of cells, and harness proton gradients to generate the energy consumed in metabolism. Indeed, of the estimated 50000–100000 genes in the human genome, fully 20–40 % are thought to encode integral membrane proteins. If one also includes membrane-associated proteins, which are attached to the membrane surface through fatty acyl chains or electrostatic interactions, this percentage is likely to be much higher.

Type
Research Article
Information
Quarterly Reviews of Biophysics , Volume 29 , Issue 4 , December 1996 , pp. 395 - 449
Copyright
Copyright © Cambridge University Press 1996

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