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From conformational chaos to robust regulation: the structure and function of the multi-enzyme RNA degradosome

Published online by Cambridge University Press:  14 December 2011

Maria W. Górna ,
Agamemnon J. Carpousis  and
Ben F. Luisi
Maria W. Górna
Affiliation:
Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, UK
Agamemnon J. Carpousis
Affiliation:
Laboratoire de Microbiologie et Génétique Moléculaires, CNRS et Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse, France
Ben F. Luisi*
Affiliation:
Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, UK
*
*Author for correspondence: Ben F. Luisi, Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, UK. Email: bfl20@mole.bio.cam.ac.uk.
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Abstract

The RNA degradosome is a massive multi-enzyme assembly that occupies a nexus in RNA metabolism and post-transcriptional control of gene expression in Escherichia coli and many other bacteria. Powering RNA turnover and quality control, the degradosome serves also as a machine for processing structured RNA precursors during their maturation. The capacity to switch between destructive and processing modes involves cooperation between degradosome components and is analogous to the process of RNA surveillance in other domains of life. Recruitment of components and cellular compartmentalisation of the degradosome are mediated through small recognition domains that punctuate a natively unstructured segment within a scaffolding core. Dynamic in conformation, variable in composition and non-essential under certain laboratory conditions, the degradosome has nonetheless been maintained throughout the evolution of many bacterial species, due most likely to its diverse contributions in global cellular regulation. We describe the role of the degradosome and its components in RNA decay pathways in E. coli, and we broadly compare these pathways in other bacteria as well as archaea and eukaryotes. We discuss the modular architecture and molecular evolution of the degradosome, its roles in RNA degradation, processing and quality control surveillance, and how its activity is regulated by non-coding RNA. Parallels are drawn with analogous machinery in organisms from all life domains. Finally, we conjecture on roles of the degradosome as a regulatory hub for complex cellular processes.

Type
Review Article
Information
Quarterly Reviews of Biophysics , Volume 45 , Issue 2 , May 2012 , pp. 105 - 145
Copyright
Copyright © Cambridge University Press 2011

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