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Fluorescence recovery after photobleaching in material and life sciences: putting theory into practice

  • Niklas Lorén (a1), Joel Hagman (a1), Jenny K. Jonasson (a2), Hendrik Deschout (a3) (a4), Diana Bernin (a5), Francesca Cella-Zanacchi (a6), Alberto Diaspro (a6), James G. McNally (a7), Marcel Ameloot (a8), Nick Smisdom (a8) (a9), Magnus Nydén (a10), Anne-Marie Hermansson (a1) (a5), Mats Rudemo (a2) and Kevin Braeckmans (a3) (a4)...

Abstract

Fluorescence recovery after photobleaching (FRAP) is a versatile tool for determining diffusion and interaction/binding properties in biological and material sciences. An understanding of the mechanisms controlling the diffusion requires a deep understanding of structure–interaction–diffusion relationships. In cell biology, for instance, this applies to the movement of proteins and lipids in the plasma membrane, cytoplasm and nucleus. In industrial applications related to pharmaceutics, foods, textiles, hygiene products and cosmetics, the diffusion of solutes and solvent molecules contributes strongly to the properties and functionality of the final product. All these systems are heterogeneous, and accurate quantification of the mass transport processes at the local level is therefore essential to the understanding of the properties of soft (bio)materials. FRAP is a commonly used fluorescence microscopy-based technique to determine local molecular transport at the micrometer scale. A brief high-intensity laser pulse is locally applied to the sample, causing substantial photobleaching of the fluorescent molecules within the illuminated area. This causes a local concentration gradient of fluorescent molecules, leading to diffusional influx of intact fluorophores from the local surroundings into the bleached area. Quantitative information on the molecular transport can be extracted from the time evolution of the fluorescence recovery in the bleached area using a suitable model. A multitude of FRAP models has been developed over the years, each based on specific assumptions. This makes it challenging for the non-specialist to decide which model is best suited for a particular application. Furthermore, there are many subtleties in performing accurate FRAP experiments. For these reasons, this review aims to provide an extensive tutorial covering the essential theoretical and practical aspects so as to enable accurate quantitative FRAP experiments for molecular transport measurements in soft (bio)materials.

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Corresponding author

* Author for correspondence: N. Lorén, Structure and Material Design, SP Food and Bioscience, PO 5401, SE-402 29, Göteborg, Sweden. Tel: +46 10 516 6614; Fax: +46 31 83 37 82; Email: niklas.loren@sp.se

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Al Tanoury, Z., Schaffner-Reckinger, E., Halavatyi, A., Hoffmann, C., Moes, M., Hadzic, E., Catillon, M., Yatskou, M. & Friederich, E. (2010). Quantitative kinetic study of the actin-bundling protein L-plastin and of its impact on actin turn-over. PLoS ONE 5, e9210.
Alvarez-Manceñido, F., Braeckmans, K., De Smedt, S. C., Demeester, J., Landin, M. & Martínez-Pacheco, R. (2006). Characterization of diffusion of macromolecules in konjac glucomannan solutions and gels by fluorescence recovery after photobleaching technique. International Journal of Pharmaceutics 316, 3746.
Arrio-Dupont, M., Foucault, G., Vacher, M., Devaux, P. F. & Cribier, S. (2000). Translational diffusion of globular proteins in the cytoplasm of cultured muscle cells. Biophysical Journal 78, 901907.
Axelrod, D. (1983). Lateral motion of membrane proteins and biological function. Journal of Membrane Biology 75, 110.
Axelrod, D., Koppel, D. E., Schlessinger, J., Elson, E. & Webb, W. W. (1976a). Mobility measurement by analysis of fluorescence photobleaching recovery kinetics. Biophysical Journal 16, 10551069.
Axelrod, D., Ravdin, P., Koppel, D. E., Schlessinger, J., Webb, W. W., Elson, E. L. & Podleski, T. R. (1976b). Lateral motion of fluorescently labeled acetylcholine receptors in membranes of developing muscle fibers. Proceedings of the National Academy of Sciences of the United States of America 73, 45944598.
Balakrishnan, G., Nicolai, T. & Durand, D. (2012). Relation between the gel structure and the mobility of tracers in globular protein gels. Journal of Colloid and Interface Science 388, 293299.
Bancaud, A., Huet, S., Daigle, N., Mozziconacci, J., Beaudouin, J. & Ellenberg, J. (2009). Molecular crowding affects diffusion and binding of nuclear proteins in heterochromatin and reveals the fractal organization of chromatin. EMBO Journal 28, 37853798.
Basser, P. J., Mattiello, J. & Lebihan, D. (1994). Estimation of the effective self-diffusion tensor from the NMR spin echo. Journal of Magnetic Resonance Series B 103, 247254.
Beaudouin, J., Mora-Bermudez, F., Klee, T., Daigle, N. & Ellenberg, J. (2006). Dissecting the contribution of diffusion and interactions to the mobility of nuclear proteins. Biophysical Journal 90, 18781894.
Betzig, E., Patterson, G. H., Sougrat, R., Lindwasser, W. O., Olenych, S., Bonifacino, J. S., Davidson, M. W., Lippincott-Schwartz, J. & Hess, H. F. (2006). Imaging intracellular fluorescent proteins at nanometer resolution. Science 313, 16421645.
Black, J. C., Cheney, P. P., Campbell, T. & Knowles, M. K. (2014). Membrane curvature based lipid sorting using a nanoparticle patterned substrate. Soft Matter 10, 20162023.
Blonk, J. C. G., Don, A., Van Aalst, H. & Birmingham, J. J. (1993). Fluorescence photobleaching recovery in the confocal scanning light microscope. Journal of Microscopy 169, 363374.
Born, M. & Wolf, E. (1999). Principles of Optics. 7th edn. Cambridge University Press, Cambridge.
Braeckmans, K., Peeters, L., Sanders, N. N., De Smedt, S. C.& Demeester, J. (2003). Three-dimensional fluorescence recovery after photobleaching with the confocal scanning laser microscope. Biophysical Journal 85, 22402252.
Braeckmans, K., Remaut, K., Vandenbroucke, R. E., Lucas, B., De Smedt, S. C. & Demeester, J. (2007). Line FRAP with the confocal laser scanning microscope for diffusion measurements in small regions of 3-D samples. Biophysical Journal 92, 21722183.
Braeckmans, K., Stubbe, B. G., Remaut, K., Demeester, J. & De Smedt, S. C. (2006). Anomalous photobleaching in fluorescence recovery after photobleaching measurements due to excitation saturation – a case study for fluorescein. Journal of Biomedical Optics 11, 044013.
Braga, J., Desterro, J. M. P. & Carmo-Fonseca, M. (2004). Intracellular macromolecular mobility measured by fluorescence recovery after photobleaching with confocal laser scanning microscopes. Molecular Biology of the Cell 15, 47494760.
Braga, J., McNally, J. G. & Carmo-Fonseca, M. (2007). A reaction-diffusion model to study RNA motion by quantitative fluorescence recovery after photobleaching. Biophysical Journal 92, 26942703.
Branco, M. C., Pochan, D. J., Wagner, N. J. & Schneider, J. P. (2009). Macromolecular diffusion and release from self-assembled β-hairpin peptide hydrogels. Biomaterials 30, 13391347.
Brandl, F., Hammer, N., Blunk, T., Tessmar, J. & Goepferish, A. (2010). Biodegradable hydrogels for time-controlled release of tethered peptides or proteins. Biomacromolecules 11, 496504.
Brown, E. B., Bouchera, Y., Nasser, S. & Jain, R. K. (2004). Measurement of macromolecular diffusion coefficients in human tumors. Microvascular Research 67, 231236.
Brown, E. B., Wu, E. S., Zipfel, W. & Webb, W. W. (1999). Measurement of molecular diffusion in solution by multiphoton fluorescence photobleaching recovery. Biophysical Journal 77, 28372849.
Brown, R. (1828). On the particles contained in the pollen of plants and on the general existence of active molecules in organic and inorganic bodies. Edinburgh New Philosophical Journal July–September, 358371.
Brown, R. (1829). Additional remarks on active molecules. Edinburgh Journal of Science 1, 314319.
Cardarelli, F., Serresi, M., Bizzarri, R., Giacca, M. & Beltram, F. (2007). In vivo study of HIV-1 Tat arginine-rich motif unveils its transport properties. Molecular Therapy 15, 13131322.
Carr, H. & Purcell, E. (1954). Effects of diffusion on free precision in nuclear magnetic resonance experiments. Physical Review 94, 630638.
Carrero, G., Crawford, E., Hendzel, M. J. & de Vries, G. (2004). Characterizing fluorescence recovery curves for nuclear proteins undergoing binding events. Bulletin of Mathematical Biology 66, 15151545.
Carvajal-Rondanelli, P. A. & Lanier, T. C. (2010). Diffusion of active proteins into fish meat to minimize proteolytic degradation. Journal of Agricultural and Food Chemistry 58, 53005307.
Cézanne, L., Lecat, S., Lagane, B., Millot, C., Vollmer, J-Y., Matthes, H., Galzi, J-L. & Lopez, A. (2004). Dynamic confinement of NK2 receptors in the plasma membrane. Improved FRAP analysis and biological relevance. Journal of Biological Chemistry 279, 4505745067.
Chaikin, P. & Lubensky, T. (1995). Principles of Condensed Matter Physics. Cambridge University Press, Cambridge.
Chen, Y., Lagerholm, B. C., Yang, B. & Jacobson, K. (2006). Methods to measure the lateral diffusion of membrane lipids and proteins. Methods 39, 147153.
Cheng, Y., Prud'homme, R. K. & Thomas, J. L. (2002). Diffusion of mesoscopic probes in aqueous polymer solutions measured by fluorescence recovery after photobleaching. Macromolecules 35, 81118121.
Cherry, R. J. (1979). Rotational and lateral diffusion of membrane proteins. Biochimica et Biophysica Acta 559, 289327.
Cherry, R. J., Smith, P. R., Morrison, I. E. & Fernandez, N. (1998). Mobility of cell surface receptors: a re-evaluation. FEBS Letters 430, 8891.
Clark, D. C., Mackie, A. R., Wilde, P. J. & Wilson, D. R. (1994). Differences in the structure and dynamics of adsorbed layers in protein-stabilized model foams and emulsions. Faraday Discussions 98, 253262.
Clark, D. C., Wilde, P. J. & Wilson, D. R. (1990). The effect of pre-isomerised hop extract on the properties of model protein stabilized foams. Journal of the Institute Brewing 97, 169172.
Costantini, L. & Snapp, E. (2013). Probing endoplasmic reticulum dynamics using fluorescence imaging and photobleaching techniques. Current Protocols in Cell Biology 60, Unit 21.7.
Crank, J. (1975). The Mathematics of Diffusion, 2nd edn. Clarendon Press, Oxford.
Cussler, E. (1997). Diffusion Mass Transfer in Fluid Systems, 2nd edn. Cambridge University Press, Cambridge.
Daddysman, M. K. & Fecko, C. J. (2013). Revisiting point FRAP to quantitatively characterize anomalous diffusion in live cells. Journal of Physical Chemistry B 117, 12411251.
Davoust, J., Devaux, P. F. & Leger, L. (1982). Fringe pattern photobleaching, a new method for the measurement of transport coefficients of biological macromolecules. EMBO Journal 1, 12331238.
Dayel, M. J., Hom, E. F. & Verkman, A. S. (1999). Diffusion of green fluorescent protein in the aqueous-phase lumen of endoplasmic reticulum. Biophysical Journal 76, 28432851.
De Clercq, B., Cleuren, B., Deschout, H., Braeckmans, K. & Ameloot, M. (2013). Distinguishing free and anomalous diffusion by rectangular fluorescence recovery after photobleaching: a Monte Carlo study. Journal of Biomedical Optics 18, 076012.
de Gennes, P.-G. (1992). Simple Views on Condensed Matter. World Scientific, Singapore.
Derichs, N., Jin, B-J., Song, Y., Finkbeiner, W. E. & Verkman, A. S. (2011). Hyperviscous airway periciliary and mucous liquid layers in cystic fibrosis measured by confocal fluorescence photobleaching. FASEB Journal 25, 23252332.
Deschout, H., Cella Zanacchi, F., Mlodzianoski, M., Diaspro, A., Bewersdorf, J., Hess, S. T. & Braeckmans, K. (2014). Precisely and accurately localizing single emitters in fluorescence microscopy. Nature Methods 11, 253266.
Deschout, H., Hagman, J., Fransson, S., Jonasson, J., Rudemo, M., Lorén, N. & Braeckmans, K. (2010). Straightforward FRAP for quantitative diffusion measurements with a laser scanning microscope. Optics Express 18, 2288622905.
Deschout, H., Raemdonck, K., Demeester, J., De Smedt, S. C. & Braeckmans, K. (2013). FRAP in pharmaceutical research: practical guidelines and applications in drug delivery. Pharmaceutical Research 31, 255270.
De Smedt, S. C., Remaut, K., Lucas, B., Braeckmans, K., Sanders, N. N. & Demeester, J. (2005). Studying biophysical barriers to DNA delivery by advanced light microscopy. Advanced Drug Delivery Reviews 57, 191210.
Dhitala, S., Shelata, K. J., Shrestha, A. K. & Gidleya, M. J. (2013). Heterogeneity in maize starch granule internal architecture deduced from diffusion of fluorescent dextran probes. Carbohydrate Polymers 93, 365373.
Diaspro, A. (2010). Nanoscopy and Multidimensional Optical Fluorescence Microscopy, Chapter 8. Chapman & Hall/CRC, Boca Raton, Florida.
Digman, M. A., Brown, C. M., Sengupta, P., Wiseman, P. W., Horwitz, A. R. & Gratton, E. (2005a). Measuring fast dynamics in solutions and cells with a laser scanning microscope. Biophysical Journal 89, 13171327.
Digman, M. A. & Gratton, E. (2009). Analysis of diffusion and binding in cells using the RICS approach. Microscopy Research and Technique 72, 323332.
Digman, M. A., Sengupta, P., Wiseman, P. W., Brown, C. M., Horwitz, A. R. & Gratton, E. (2005b). Fluctuation correlation spectroscopy with a laser-scanning microscope: exploiting the hidden time structure. Biophysical Journal 88, L33L36.
Dill, K. & Bromberg, S. (2003). Molecular Driving Forces. Garland Science, London.
Dix, J. A. & Verkman, A. S. (2008). Crowding effects on diffusion in solutions and cells. Annual Review in Biophysics 37, 247263.
Dushek, O., Das, R. & Coombs, D. (2008). Analysis of membrane-localized binding kinetics with FRAP. European Biophysics Journal 37, 627638.
Edward, J. (1970). Molecular volumes and the Stokes-Einstein equation. Journal of Chemical Education 47, 261270.
Einstein, A. (1905). Über die von der molekularkinetischen Theorie der Wärme geforderte Bewegung von in ruhenden Flüssigkeiten suspendierten Teilchen. Annalen der Physik 17, 549560.
Endress, E., Wiegelt, S., Reents, G. & Bayer, T. M. (2005). Derivation of a closed form analytical expression for fluorescence recovery after photobleaching in the case of continuous bleaching during readout. The European Physics Journal E 16, 8187.
Erdel, F. & Rippe, K. (2012). Quantifying transient binding of ISWI chromatin remodelers in living cells by pixel-wise photobleaching profile evolution analysis. Proceedings of the National Academy of Sciences of the United States of America 109, E3221E3230.
Feder, T. J., Brust-Mascher, I., Slattery, J. P., Baird, B. & Webb, W. W. (1996). Constrained diffusion or immobile fraction on cell surfaces: a new interpretation. Biophysical Journal 70, 27672773.
Fick, A. (1855). Über diffusion. Annalen der Physik und Chemie 94, 5986.
Floury, J., Madec, M.-N., Waharte, F., Jeanson, S. & Lortal, S. (2012). First assessment of diffusion coefficients in model cheese by fluorescence recovery after photobleaching (FRAP). Food Chemistry 133, 551556.
Frye, L. D. & Edidin, M. (1970). The rapid intermixing of cell surface antigens after formation of mouse-human heterokaryons. Journal of Cell Science 7, 319335.
García-Sáez, A. J. & Schwille, P. (2010). Surface analysis of membrane dynamics. Biochimica et Biophysica Acta 1798, 766776.
Ghosh, V., Ziegler, G. R. & Anantheswaran, R. C. (2002). Fat, moisture and ethanol migration through chocolates and confectionary coatings. Critical Reviews in Food Science and Nutrition 42, 583626.
Gonzalez-Gonzalez, I. M., Jaskolski, F., Goldberg, Y., Ashby, M. C. & Henley, J. M. (2012). Measuring membrane protein dynamics in neurons using fluorescence recovery after photobleach. Methods in Enzymology 504, 127146.
González-Pérez, V., Schmierer, B., Hill, C. S. & Sear, R. P. (2011). Studying Smad2 intranuclear diffusion dynamics by mathematical modelling of FRAP experiments. Integrative Biology 3, 197207.
Gordon, G., Chazotte, B., Wang, X. F. & Herman, B. (1995). Analysis of simulated and experimental fluorescence recovery after photobleaching data for two diffusing components. Biophysical Journal 68, 766778.
Green, P. (2005). Kinetics, Transport and Structure in Hard and Soft Materials. CRC Press, New York.
Groeneweg, F. L., van Royen, M. E., Fenz, S., Keizer, V. I., Geverts, B., Prins, J., de Kloet, E. R., Houtsmuller, A. B., Schmidt, T. S. & Schaaf, M. J. (2014). Quantitation of glucocorticoid receptor DNA-binding dynamics by single-molecule microscopy and FRAP. PLoS ONE 9(3): e90532.
Guizar-Sicairos, M. & Gutierrez-Vega, J. C. (2004). Computation of quasi-discrete Hankel transforms of integer order for propagating optical wave fields. Journal of Optical Society of America A 21, 5358.
Hager, G. L., McNally, J. G. & Misteli, T. (2009). Transcription dynamics. Molecular Cell 35, 741753.
Hagman, J., Lorén, N. & Hermansson, A.-M. (2010). Effect of gelatin gelation kinetics on probe diffusion determined by FRAP and rheology. Biomacromolecules 11, 33593366.
Hagman, J., Lorén, N. & Hermansson, A.-M. (2012). Probe diffusion in κ-carrageenan gels determined by fluorescence recovery after photobleaching. Food Hydrocolloids 29, 106115.
Haj, F. G., Sabet, O., Kinkhabwala, A., Wimmer-Kleikamp, S., Roukos, V., Han, H. M., Grabenbauer, M., Bierbaum, M., Antony, C., Neel, B. G. & Bastiaens, P. I. (2012). Regulation of signaling at regions of cell-cell contact by endoplasmic reticulum-bound protein-tyrosine phosphatase 1B. PLoS ONE 7, e36633.
Hallen, M. A., Ho, J., Yankel, C. D. & Endow, S. A. (2008a). Fluorescence recovery kinetic analysis of gamma-tubulin binding to the mitotic spindle. Biophysical Journal 95, 30483058.
Hallen, M. A. & Layton, A. T. (2010). Expanding the scope of quantitative FRAP analysis. Journal of Theoretical Biology 262, 295305.
Hallen, M. A., Liang, Z. Y. & Endow, S. A. (2008b). Ncd motor binding and transport in the spindle. Journal of Cell Science 121, 38343841.
Hansen, J.-P. & McDonald, R. (2006). Theory of Simple Liquids, 3rd edn. Elsevier, Amsterdam.
Hardy, L. R. (2012). Fluorescence recovery after photobleaching (FRAP) with a focus on F-actin. Current Protocol in Neurosciences, Chapter 2: Unit 2.17.
Hawlicka, E. (1995). Self-diffusion in multicomponent liquid systems. Chemical Society Reviews 24, 367377.
Hebert, B., Costantino, S. & Wiseman, P. W. (2005). Spatiotemporal image correlation spectroscopy (STICS) theory, verification, and application to protein velocity mapping in living CHO cells. Biophysical Journal 88, 36013614.
Heitjans, P. & Kärger, J. (2005). Diffusion in Condensed Matter. Berlin, Heidelberg: Springer-Verlag.
Hell, S. W. & Wichmann, J. (1994). Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy. Optics Letters 19, 780782.
Hellriegel, C., Kirstein, J., Brauchle, C., Latour, V., Pigot, T., Olivier, R., Lacombe, S., Brown, R., Guieu, V. & Payrastre, C. (2004). Diffusion of single streptocyanine molecules in the nanoporous network of sol–gel glasses. Journal of Physical Chemistry B 108, 1469914709.
Hellriegel, C., Kirstein, J. & Brauchle, C. (2005). Tracking of single molecules as a powerful method to characterize diffusivity of organic species in mesoporous materials. New Journal of Physics 7(23): 114.
Herman, B. (1998). Fluorescence Microscopy. UK: BIOS Scientific Publishers, Royal Microscopical Society.
Hermansson, A.-M., Lorén, N. & Nydén, M. (2006). The importance of microstructure for solvent and solute diffusion on the micro and nano length scales. In Water Properties of Food, Pharmaceutical, and Biological Materials (eds. del Pilar Buera, M., Welti-Chanes, J., Lillford, P. J. & Corti, H. R.), p. 79. CRC Taylor and Francis, Boca Raton.
Hinow, P., Rogers, C. E., Barbieri, C. E., Pietenpol, J. A., Kenworthy, A. K. & DiBenedetto, E. (2006). The DNA binding activity of p53 displays reaction-diffusion kinetics. Biophysical Journal 91, 330342.
Holmberg, C. I., Staniszewski, K. E., Mensah, K. N., Matouschek, A. & Morimoto, R. I. (2004). Inefficient degradation of truncated polyglutamine proteins by the proteasome. EMBO Journal 23, 43074318.
Houtsmuller, A. B. (2005). Fluorescence recovery after photobleaching: application to nuclear proteins. Advances in Biochemical Engineering/Biotechnology 95, 177199.
Inoué, S. (2006). Foundations of confocal scanned imaging in light microscopy. In Biological Confocal Microscopy, Chapter 1 (ed. Pawley, J. B.), pp. 119. Springer Science+Business Media, New York.
Jensen, S., Rolin, C. & Ipsen, R. (2010). Stabilisation of acidified skimmed milk with HM pectin. Food Hydrocolloids 24, 291299.
Jonasson, J. K., Hagman, J., Lorén, N., Bernin, D., Nydén, M. & Rudemo, M. (2010). Pixel-based analysis of FRAP data with a general initial bleaching profile. Journal of Microscopy 239, 142153.
Jonasson, J. K., Lorén, N., Olofsson, P., Nydén, M. & Rudemo, M. (2008). A pixel-based likelihood framework for analysis of fluorescence recovery after photobleaching data. Journal of Microscopy 232, 260269.
Jonkman, J. E. N. & Stelzer, E. H. K. (2002). Resolution and contrast in confocal and two-photon microscopy. In Confocal and Two-Photon Microscopy, Chapter 5 (ed. Diaspro, A.), pp. 101125. New York, USA: Wiley-Liss, Inc.
Jönsson, B., Wennerström, H., Nilsson, P. G. & Linse, P. (1986). Self-diffusion of small molecules in colloidal systems. Colloid and Polymer Science 264, 7788.
Jönsson, P., Jonsson, M. P., Tegenfeldt, J. O. & Höök, F. (2008). A method improving the accuracy of fluorescence recovery after photobleaching analysis. Biophysical Journal 95, 53345348.
Kang, M., Day, C. A., DiBenedetto, E. & Kenworthy, A. K. (2010). A quantitative approach to analyze binding diffusion kinetics by confocal FRAP. Biophysical Journal 99, 27372747.
Kang, M., Day, C. A., Drake, K., Kenworthy, A. K. & DiBenedetto, E. A. (2009). A generalization of theory for two-dimensional fluorescence recovery after photobleaching applicable to confocal laser scanning microscopy. Biophysical Journal 97, 15011511.
Kang, M., DiBenedetto, E. & Kenworthy, A. K. (2011). Proposed correction to Feder's anomalous diffusion FRAP equations. Biophysical Journal 100, 791792.
Kapitza, H. G., Mcgregor, G. & Jacobson, K. A. (1985). Direct measurement of lateral transport in membranes by using time-resolved spatial photometry. Proceedings of the National Academy of Sciences of the United States of America 82, 41224126.
Karbowiak, T., Debeaufort, F., Voilley, A. & Trystram, G. (2009). From macroscopic to molecular scale investigations of mass transfer of small molecules through edible packaging applied at interfaces of multiphase food products. Innovative Food Science and Emerging Technologies 10, 116127.
Karbowiak, T., Hervet, H., Léger, L., Champion, D., Debeaufort, F. & Voilley, A. (2006). Effect of plasticizers (water and glycerol) on the diffusion of a small molecule in iota-carrageenan biopolymer films for edible coating application. Biomacromolecules 7, 20112019.
Kaufman, E. N. & Jain, R. K. (1990). Quantification of transport and binding parameters using fluorescence recovery after photobleaching. Potential for in vivo applications. Biophysical Journal 58, 873885.
Kenworthy, A. K. (2007). Fluorescence recovery after photobleaching studies of lipid rafts. Methods in Molecular Biology 398, 179192.
Kenworthy, A. K., Nichols, B. J., Remmert, C. L., Hendrix, G. M., Kumar, M., Zimmerberg, J. & Lippincott-Schwartz, J. (2004). Dynamics of putative raft-associated proteins at the cell surface. Journal of Cell Biology 165, 735746.
Kolin, D. L. & Wiseman, P. V. (2007). Advances in image correlation spectroscopy: measuring number densities, aggregation states, and dynamics of fluorescently labeled macromolecules in cells. Cell Biochemistry and Biophysics 49, 141164.
Koppel, D. E., Axelrod, D., Schlessinger, J., Elson, E. L. & Webb, W. W. (1976). Dynamics of fluorescence marker concentration as a probe of mobility. Biophysical Journal 16, 13151329.
Koster, M., Frahm, T. & Hauser, H. (2005). Nucleocytoplasmic shuttling revealed by FRAP and FLIP technologies. Current Opinion in Biotechnology 16, 2834.
Kubitscheck, U., Wedekind, P. & Peters, R. (1994). Lateral diffusion measurement at high spatial resolution by scanning microphotolysis in a confocal microscope. Biophysical Journal 67, 948956.
Kubitscheck, U., Wedekind, P. & Peters, R. (1998). Three-dimensional diffusion measurements by scanning microphotolysis. Journal of Microscopy 192, 126138.
Kusto, K. B. & Deen, W. M. (2004). Diffusivities of macromolecules in composite hydrogels. AiChe Journal 50, 26482658.
Kusumi, A., Koyama-Honda, I. & Suzuki, K. (2004). Molecular dynamics and interactions for creation of stimulation-induced stabilized rafts from small unstable steady-state rafts. Traffic 5, 213230.
Latour, L. L., Mitra, P. P., Kleinberg, R. L. & Sotak, C. H. (1993). Time-dependent diffusion coefficient of fluids in porous media as a probe of surface-to-volume ratio. J. Magn. Reson. Series A 101, 342346.
Lele, T., Oh, P., Nickerson, J. A. & Ingber, D. E. (2004). An improved mathematical approach for determination of molecular kinetics in living cells with FRAP. Mechanics & Chemistry of Biosystems 1, 181190.
Lenk, R. (1986). Fluctuations, Diffusion and Spin Relaxation. Elsevier, New York.
Levi, V. & Gratton, E. (2007). Exploring dynamics in living cells by tracking single particles. Cell Biochemistry and Biophysics 48, 115.
Lin, C. C. & Metters, A. T. (2006). Hydrogels in controlled release formulations: network design and mathematical modeling. Advanced Drug Delivery Reviews 58, 13791408.
Lippincott-Schwartz, J., Altan-Bonnet, N. & Patterson, G. H. (2003). Photobleaching and photoactivation: following protein dynamics in living cells. Nature Cell Biology 5, S7S14.
Lippincott-Schwartz, J. & Patterson, G. H. (2003). Development and use of fluorescent protein markers in living cells. Science 300, 8791.
Lorén, N., Nydén, M. & Hermasson, A-M. (2009). Determination of local diffusion properties in heterogeneous biomaterials. Advances in Colloidal and Interface Science 150, 515.
Lubelski, A. & Klafter, J. (2008). Fluorescence recovery after photobleaching: the case of anomalous diffusion. Biophysical Journal 94, 46464653.
Lukacs, G. L., Haggie, P., Seksek, O., Lechardeur, D., Freedman, N. & Verkman, A. S. (2000). Size-dependent DNA mobility in cytoplasm and nucleus. Journal of Biological Chemistry 275, 16251629.
Lukyanov, K. A., Chudakov, D. M., Lukyanov, S. & Verkhusha, V. V. (2005). Innovation: photoactivatable fluorescent proteins. Nature Reviews Molecular Cell Biology 6, 88589.
Luu, D. T. & Maurel, C. (2013). Aquaporin trafficking in plant cells: an emerging membrane-protein model. Traffic 14, 629635.
Mackie, A. R., Nativel, S., Wilson, D. R., Ladha, S. & Clark, D. C. (1996). Process-induced changes in molecular structure that alter adsorbed layer properties in oil-in-water emulsions stabilised by β-Casein/Tween20 mixtures. Journal of the Science of Food and Agriculture 70, 413421.
Marguet, D., Lenne, P. F., Rigneault, H. & He, H. T. (2006). Dynamics in the plasma membrane: how to combine fluidity and order. EMBO Journal 25, 34463457.
Marty, S., Schroeder, M., Baker, K. W., Mazzanti, G. & Marangoni, A. G. (2009). Small molecule diffusion through polycrystalline triglyceride networks quantified using fluorescent recovery after photobleaching. Langmuir 25, 87808785.
Maru, B. S., Tobias, J. H., Rivers, C., Caunt, C. J., Normana, M. R. & McArdlea, C. A. (2009). Potential use of an estrogen–glucocorticoid receptor chimera as a drug screen for tissue selective estrogenic activity. Bone 44, 102112.
Maxfield, F. R., Willingham, M. C., Pastan, I., Dragsten, P. & Cheng, S. Y. (1981). Binding and mobility of the cell surface receptors for 3,3′,5-triiodo-L-thyronine. Science 211, 6365.
Mazza, D., Abernathy, A., Golob, N., Morisaki, T. & McNally, J. G. (2012). A benchmark for chromatin binding measurements in live cells. Nucleic Acids Research 40, e119.
Mazza, D., Braeckmans, K., Cella, F., Testa, I., Vercauteren, D., Demeester, J., De Smedt, S. S. & Diaspro, A. (2008). A new FRAP/FRAPa method for three-dimensional diffusion measurements based on multiphoton excitation microscopy. Biophysical Journal 95, 34573469.
Mazza, D., Cella, F., Vicidomini, G., Krol, S. & Diaspro, A. (2007). Role of three-dimensional bleach distribution in confocal and two-photon fluorescence recovery after photobleaching experiments. Applied Optics 46, 74017411.
McGrath, J. L., Tardy, Y., Dewey, C. F., Meister, J. J. & Hartwig, J. H. (1998). Simultaneous measurements of actin filament turnover, filament fraction, and monomer diffusion in endothelial cells. Biophysical Journal 75, 20702078.
Meder, D., Moreno, M. J., Verkade, P., Vaz, W. L. & Simons, K. (2006). Phase coexistence and connectivity in the apical membrane of polarized epithelial cells. Proceedings of the National Academy of Sciences of the United States of America 103, 329334.
Meyvis, T. K., De Smedt, S. C., Van Oostveldt, P. & Demeester, J. (1999). Fluorescence recovery after photobleaching: a versatile tool for mobility and interaction measurements in pharmaceutical research. Pharmaceutical Research 16, 11531162.
Michelman-Ribeiro, A., Mazza, D., Rosales, T., Stasevich, T. J., Boukari, H., Rishi, V., Vinson, C., Knutson, J. R. & McNally, J. G. (2009). Direct measurement of association and dissociation rates of DNA binding in live cells by fluorescence correlation spectroscopy. Biophysical Journal 97, 337346.
Minati, L. & Weglarz, W. (2007). Physical foundations, models, and methods of diffusion magnetic resonance imaging of the brain: a review. Concepts in Magnetic Resonance 30A, 278307.
Montero Llopis, P., Sliusarenko, O., Heinritz, J. & Jacobs-Wagner, C. (2012). In vivo biochemistry in bacterial cells using FRAP: insight into the translation cycle. Biophysical Journal 103, 18481859.
Mori, S. (2007). Introduction to Diffusion Tensor Imaging. Oxford: Elsevier.
Morisaki, T. & McNally, J. G. (2014). Photoswitching-free FRAP analysis with a genetically encoded fluorescent tag. PLoS ONE 18, 9(9), e107730.
Mueller, F., Mazza, D., Stasevich, T. J. & McNally, J. G. (2010). FRAP and kinetic modeling in the analysis of nuclear protein dynamics: what do we really know? Current Opinion in Cell Biology 22, 403411.
Mueller, F., Morisaki, T., Mazza, D. & McNally, J. G. (2012). Minimizing the impact of photoswitching of fluorescent proteins on FRAP analysis. Biophysical Journal 102, 16561665.
Mueller, F., Wach, P. & McNally, J. G. (2008). Evidence for a common mode of transcription factor interaction with chromatin as revealed by improved quantitative fluorescence recovery after photobleaching. Biophysical Journal 94, 33233339.
Müller, K. P., Erdel, F., Caudron-Herger, M., Marth, C., Fodor, B. D., Richter, M., Scaranaro, M., Beaudouin, J., Wachsmuth, M. & Rippe, K. (2009). Multiscale analysis of dynamics and interactions of heterochromatin protein 1 by fluorescence fluctuation microscopy. Biophysical Journal 97, 28762885.
Nickerson, J. A. (2009). The biochemistry of RNA metabolism studied in situ. RNA Biology 6, 2530.
Netti, P. A., Berk, D. A., Swartz, M. A., Grodzinsky, A. J. & Jain, R. K. (2000). Role of extracellular matrix assembly in interstitial transport in solid tumors. Cancer Research 60, 24972503.
Norris, S. C., Humpolíčková, J., Amler, E., Huranová, M., Buzgo, M., Macháň, R., Lukáš, D. & Hof, M. (2011). Raster image correlation spectroscopy as a novel tool to study interactions of macromolecules with nanofiber scaffolds. Acta Biomaterialia 7, 41954203.
Notelaers, K., Smisdom, N., Rocha, S., Janssen, D., Meier, J. C., Rigo, J. M., Hofkens, J. & Ameloot, M. (2012). Ensemble and single particle fluorimetric techniques in concerted action to study the diffusion and aggregation of the glycine receptor α3 isoforms in the cell plasma membrane. Biochimica et Biophysica Acta 1818, 31313140.
Orlova, D. Y., Bartova, E., Maltsev, V. P., Kozubek, S. & Chernyshev, A. V. (2011). A nonfitting method using a spatial sine window transform for inhomogeneous effective-diffusion measurements by FRAP. Biophysical Journal 100, 507516.
Packer, K. J., Rees, C. & Tomlinson, D. J. (1972). Studies of diffusion and flow by pulsed NMR techniques. Advances in Molecular Relaxation Processes 3, 119131.
Papadopoulos, M. C., Binder, D. K. & Verkman, A. S. (2005). Enhanced macromolecular diffusion in brain extracellular space in mouse models of vasogenic edema measured by cortical surface photobleaching. FASEB Journal 19, 425427.
Pastor, I., Vilaseca, E., Madurga, S., Lluís Garcés, J., Cascante, M. & Mas, F. (2010). Diffusion of α-Chymotrypsin in solution-crowded media. A fluorescence recovery after photobleaching study. Journal of Physical Chemistry B 114, 40284034.
Patterson, G. H. & Piston, D. W. (2000). Photobleaching in two-photon excitation microscopy. Biophysical Journal 78, 21592162.
Pawley, J. B. (2006). Handbook of Biological Confocal Microscopy, 3rd edn. New York: Springer.
Payet, L., Ponton, A., Léger, L., Hervet, H., Grossiord, J. L. & Agnely, F. (2008). Self-diffusion in chitosan networks: from a gel-gel method to fluorescence recovery after photobleaching by fringe pattern. Macromolecules 41, 93769381.
Periasamy, N. & Verkman, A. S. (1998). Analysis of fluorophore diffusion by continuous distributions of diffusion coefficients: application to photobleaching measurements of multicomponent and anomalous diffusion. Biophysical Journal 75, 557567.
Peters, R., Peters, J., Tews, K. H. & Bähr, W. (1974). A microfluorimetric study of translational diffusion in erythrocyte membranes. Biochimica et Biophysica Acta 367, 282294.
Petersen, N. O., Hoddelius, P. L., Wiseman, P. W., Seger, O. & Magnusson, K. E. (1993). Quantitation of membrane receptor distributions by image correlation spectroscopy: concept and application. Biophysical Journal 65, 11351146.
Phair, R. D. & Misteli, T. (2000). High mobility of proteins in the mammalian cell nucleus. Nature 404, 604609.
Phair, R. D. & Misteli, T. (2001). Kinetic modelling approaches to in vivo imaging. Nature Reviews Molecular Cell Biology 2, 898907.
Pike, L. J. (2006). Rafts defined: a report on the Keystone Symposium on Lipid Rafts and Cell Function. Journal of Lipid Research 47, 15971598.
Pinte, J., Joly, C., Plé, K., Dole, P. & Feigenbaum, A. (2008). Proposal of a set of model polymer additives designed for confocal FRAP diffusion experiments. Journal of Agricultural and Food Chemistry 56, 1000310011.
Pluen, A., Boucher, Y., Ramanujan, S., McKee, T. D., Gohongi, T., di Tomaso, E., Brown, E. B., Izumi, Y., Campbell, R. B., Berk, D. A. & Jain, R. K. (2001). Role of tumor–host interactions in interstitial diffusion of macromolecules: cranial vs. subcutaneous tumors. Proceedings of the National Academy of Sciences of the United States of America 98, 46224633.
Price, W. (1997). Pulsed-field gradient nuclear magnetic resonance as a tool for studying translational diffusion: Part 1. Basic theory. Concepts in Magnetic Resonance 9, 299336.
Price, W. (2009). NMR Studies of Translational Motion. Cambridge University Press, Cambridge.
Raghuram, N., Carrero, G., Th'ng, J. & Hendzel, M. J. (2009). Molecular dynamics of histone H1. Biochemistry and Cell Biology 87, 189206.
Reits, E., Griekspoor, A., Neijssen, J., Groothuis, T., Jalink, K., van Veelen, P., Janssen, H., Calafat, J., Drijfhout, J. W. & Neefjes, J. (2003). Peptide diffusion, protection, and degradation in nuclear and cytoplasmic compartments before antigen presentation by MHC class I. Immunity 18, 97108.
Remaut, K., Sanders, N. N., De Geest, B. G., Braeckmans, K., Demeester, J. & De Smedt, S. C. (2007). Nucleic acid delivery: where material sciences and bio-sciences meet. Materials Science & Engineering R – Reports 58, 117161.
Renz, M. & Langowski, J. (2008). Dynamics of the CapG actin-binding protein in the cell nucleus studied by FRAP and FCS. Chromosome Research 16, 427437.
Ritchie, K., Shan, X. Y., Kondo, J., Iwasawa, K., Fujiwara, T. & Kusumi, A. (2005). Detection of non-Brownian diffusion in the cell membrane in single molecule tracking. Biophysical Journal 88, 22662277.
Rossow, M. J., Sasaki, J. M., Digman, M. A. & Gratton, E. (2010). Raster image correlation spectroscopy in live cells. Nature Protocols 5, 17611774.
Ruthardt, N., Lamb, D. C. & Brauchle, C. (2011). Single-particle tracking as a quantitative microscopy-based approach to unravel cell entry mechanisms of viruses and pharmaceutical nanoparticles. Molecular Therapy: The Journal of the American Society of Gene Therapy 19, 11991211.
Sadegh, Z. K. & Montas, H. J. (2010). A class of exact solutions for biomacromolecule diffusion-reaction in live cells. Journal of Theoretical Biology 264, 914933.
Saffman, P. G. & Delbruck, M. (1975). Brownian motion in biological membranes. Proceedings of the National Academy of Sciences of the United States of America 72, 31113113.
Salinas, D., Haggie, P. M., Thiagarajah, J. R., Song, Y., Rosbe, K., Finkbeiner, W. E., Nielson, D. W. & Verkman, A. S. (2005). Submucosal gland dysfunction as a primary defect in cystic fibrosis. FASEB Journal 19, 431433.
Salome, L., Cazeils, J. L., Lopez, A. & Tocanne, J. F. (1998). Characterization of membrane domains by FRAP experiments at variable observation areas. European Biophysical Journal 27, 391402.
Sanchez, S. A. & Gratton, E. (2005). Lipid–protein interactions revealed by two-photon microscopy and fluorescence correlation spectroscopy. Accounts of Chemical Research 38, 469477.
Sanger, J. W., Wang, J., Fan, Y., White, J. & Sanger, J. M. (2010). Assembly and dynamics of myofibrils. Journal of Biomedicine and Biotechnology 2010, 858606.
Saxton, M. J. (1996). Anomalous diffusion due to binding: a Monte Carlo study. Biophysical Journal 70, 12501262.
Saxton, M. J. (1997). Single-particle tracking: the distribution of diffusion coefficients. Biophysical Journal 72, 17441753.
Saxton, M. J. (2001). Anomalous subdiffusion in fluorescence photobleaching recovery: a Monte Carlo study. Biophysical Journal 81, 22262240.
Saxton, M. J. (2007). A biological interpretation of transient anomalous subdiffusion, I. Qualitative model. Biophysical Journal 92, 11781191.
Saxton, M. J. & Jacobson, K. (1997). Single-particle tracking: applications to membrane dynamics. Annual Review of Biophysics and Biomolecular Structure 26, 373399.
Saxton, W. M., Stemple, D. L., Leslie, R. J., Salmon, E. D., Zavortink, M. & McIntosh, J. R. (1984). Tubulin dynamics in cultured mammalian cells. Journal of Cell Biology 99, 21752186.
Sbalzarini, I. F., Mezzacasa, A., Helenius, A. & Koumoutsakos, P. (2005). Effects of organelle shape on fluorescence recovery after photobleaching. Biophysical Journal 89, 14821492.
Schlessinger, J., Elson, E. L., Webb, W. W., Yahara, I., Rutishauser, U., Edelman, G. M. (1977). Receptor diffusion on cell surfaces modulated by locally bound concanavalin A. Proceedings of the National Academy of Sciences of the United States of America 74, 11101114.
Schlessinger, J., Shechter, Y., Cuatrecasas, P., Willingham, M. C. & Pastan, I. (1978). Quantitative determination of the lateral diffusion coefficients of the hormone-receptor complexes of insulin and epidermal growth factor on the plasma membrane of cultured fibroblasts. Proceedings of the National Academy of Sciences of the United States of America 75, 53535357.
Schlessinger, J., Webb, W. W., Elson, E. L. & Metzger, H. (1976). Lateral motion and valence of Fc receptors on rat peritoneal mast cells. Nature 264, 550552.
Schmiedeberg, L., Weisshart, K., Diekmann, S., Meyer, Z., Hoerste, G. & Hemmerich, P. (2004). High- and low-mobility populations of HP1 in heterochromatin of mammalian cells. Molecular Biology of the Cell 15, 28192833.
Schuster, E., Eckardt, J., Hermansson, A-M., Larsson, A., Lorén, N., Altskär, A. & Ström, A. (2014a). Microstructural, mechanical and mass transport properties of isotropic and capillary alginate gels. Soft Matter 10, 357366.
Schuster, E., Hermansson, A-M., Öhgren, C., Rudemo, M. & Lorén, N. (2014b). Interactions and diffusion in fine-stranded beta-lactoglobulin gels determined via FRAP and binding. Biophysical Journal 106, 253262.
Shaner, N. C., Patterson, G. H. & Davidson, M. W. (2007). Advances in fluorescent protein technology. Journal of Cell Science 120, 42474260.
Shelat, K. J., Vilaplana, F., Nicholson, T. M., Wonga, K. H., Gidley, M. J. & Gilbert, R. G. (2010). Diffusion and viscosity in arabinoxylan solutions: implications for nutrition. Carbohydrate Polymers 82, 4653.
Shen, H., Hu, Y. & Saltzman, W. M. (2006). DNA diffusion in mucus: effect of size, topology of DNAs, and transfection reagents. Biophysical Journal 91, 639644.
Shi, C., Cisewski, S. E., Bell, P. D. & Yao, H. (2014). Measurement of three-dimensional anisotropic diffusion by multiphoton fluorescence recovery after photobleaching. Annals of Biomedical Engineering 42, 555565.
Siggia, E. D., Lippincott-Schwartz, J. & Bekiranov, S. (2000). Diffusion in inhomogeneous media: theory and simulations applied to whole cell photobleach recovery. Biophysical Journal 79, 17611770.
Silva, J. V. C., Peixoto, P. D. S., Lortal, S. & Floury, J. (2013). Transport phenomena in a model cheese: the influence of the charge and shape of solutes on diffusion. Journal of Dairy Science 96, 61866198.
Singer, J. (1978). NMR diffusion and flow measurements and an introduction to spin phase graphing. Journal of Physics E 11, 281291.
Singer, S. J. & Nicolson, G. L. (1972). The fluid mosaic model of the structure of cell membranes. Science 175, 720731.
Sinnecker, D., Voigt, P., Hellwig, N. & Schaefer, M. (2005). Reversible photobleaching of enhanced green fluorescent proteins. Biochemistry 44, 70857094.
Smisdom, N., Braeckmans, K., Deschout, H., van de Ven, M., Rigo, J.-M., De Smedt, S. C. & Ameloot, M. (2011). Fluorescence recovery after photobleaching on the confocal laser-scanning microscope: generalized model without restriction on the size of the photobleached disk. Journal of Biomedical Optics 16, 046021.
Smith, B. A. & McConnell, H. M. (1978). Determination of molecular motion in membranes using periodic pattern photobleaching. Proceedings of the National Academy of Sciences of the United States of America 75, 27592763.
Smoluchowski, M. (1906). Zur kinetischen Theorie der Brownsche Molekularbewegung und der Suspensionen. Annalen der Physik 21, 756780.
Snieckers, Y. H. & van Donkelaar, C. C. (2005). Determining diffusion coefficients in inhomogeneous tissues using fluorescence recovery after photobleaching. Biophysical Journal 89, 13021307.
Soumpasis, D. M. (1983). Theoretical analysis of fluorescence photobleaching recovery experiments. Biophysical Journal 41, 9597.
Sprague, B. L. & McNally, J. G. (2005). FRAP analysis of binding: proper and fitting. Trends in Cell Biology 15, 8491.
Sprague, B. L., Muller, F., Pego, R. L., Bungay, P. M., Stavreva, D. A. & McNally, J. G. (2006). Analysis of binding at a single spatially localized cluster of binding sites by fluorescence recovery after photobleaching. Biophysical Journal 91, 11691191.
Sprague, B. L., Pego, R. L., Stavreva, D. A. & McNally, J. G. (2004). Analysis of binding reactions by fluorescence recovery after photobleaching. Biophysical Journal 86, 34733495.
Srivastava, M. & Petersen, N. O. (1998). Diffusion of transferrin receptor clusters. Biophysical Chemistry 75, 201211.
Stasevich, T. J., Mueller, F., Brown, D. T. & McNally, J. G. (2010a). Dissecting the binding mechanism of the linker histone in live cells: an integrated FRAP analysis. EMBO Journal 29, 12251234.
Stasevich, T. J., Mueller, F., Michelman-Ribeiro, A., Rosales, T., Knutson, J. R. & McNally, J. G. (2010b). Cross-validating FRAP and FCS to quantify the impact of photobleaching on binding measurements in live cells. Biophysical Journal 29, 12251234.
Stenoien, D. L., Mielke, M. & Mancini, M. A. (2002). Intranuclear ataxin1 inclusions contain both fast- and slow-exchanging components. Nature Cell Biology 4, 806810.
Stokes, G. (1856). On the effect of the internal friction of fluids on the motion of pendulums. Transactions of the Cambridge Philosophical Society 9, 8106.
Stylianopoulos, T., Diop-Frimpong, B., Munn, L. L. & Jain, R. K. (2010). Diffusion anisotropy in collagen gels and tumors: the effect of fiber network orientation. Biophysical Journal 99, 31193128.
Suh, J., Dawson, M. & Hanes, J. (2005). Real-time multiple-particle tracking: applications in drug/gene delivery. Advanced Drug Delivery Reviews 57, 6378.
Sullivan, K. D. & Brown, E. B. (2011). Multiphoton fluorescence recovery after photobleaching in bounded systems. Physical Review E 83, 051916.
Sullivan, K, D., Sipprell, W. H., Brown, E. B. Jr. & Brown, E. B. (2009). Improved model of fluorescence recovery expands the application of multiphoton fluorescence recovery after photobleaching in vivo . Biophysical Journal 96, 50825094.
Svanberg, L., Ahrné, L., Lorén, N. & Windhab, E. (2011). Effect of pre-crystallization process and solid particle addition on compactness of structure in chocolate model systems. Food Research International 44, 13391350.
Swaminathan, R., Hoang, C. P. & Verkman, A. S. (1997). Photobleaching recovery and anisotropy decay of green fluorescent protein GFP-S65T in solution and cells: cytoplasmic viscosity probed by green fluorescent protein translational and rotational diffusion. Biophysical Journal 72, 19001907.
Travascio, F. & Gu, W. Y. (2011). Simultaneous measurement of anisotropic solute diffusivity and binding reaction rates in biological tissues by FRAP. Annals of Biomedical Engineering 39, 5365.
Trembacka, D. O., Kuzak, M. & Dobrucki, J. W. (2010). Conditions for using FRAP as a quantitative technique – influence of the bleaching protocol. Cytometry A 77A, 366370.
Tsay, T.-T. & Jacobson, K. A. (1991). Spatial Fourier analysis of video photobleaching measurements – principles and optimization. Biophysical Journal 60, 360368.
Tsibidis, G. D. (2009). Quantitative interpretation of binding reactions of rapidly diffusing species using fluorescence recovery after photobleaching. Journal of Microscopy 233, 384390.
Tsibidis, G. D. & Ripoll, J. (2008). Investigation of binding mechanisms of nuclear proteins using confocal scanning laser microscopy and FRAP. Journal of Theoretical Biology 253, 755768.
Tsien, R. Y. (1998). The green fluorecsent protein. Annual Review of Biochemistry 67, 50944.
Van Oostveldt, P. & Bauwens, S. (1990). Quantitative fluorescence in confocal microscopy. The effect of the detection pinhole aperture on the re-absorption and inner filter phenomena. Journal of Microscopy 158, 121132.
van Royen, M. E., Farla, P., Mattern, K. A., Geverts, B., Trapman, J. & Houtsmuller, A. B. (2009). Fluorescence recovery after photobleaching (FRAP) to study nuclear protein dynamics in living cells. Methods in Molecular Biology 464, 363385.
Verheyen, E., van der Wal, S., Deschout, H., Braeckmans, K., de Smedt, S., Barendregt, A., Hennink, W. E. & van Nostrum, C. F. (2011). Protein macromonomers containing reduction-sensitive linkers for covalent immobilization and glutathione triggered release from dextran hydrogels. Journal of Controlled Release 156, 329336.
Videcoq, P., Steenkeste, K., Bonnina, E. & Garnier, C. (2013). A multi-scale study of enzyme diffusion in macromolecular solutions and physical gels of pectin polysaccharides. Soft Matter 9, 5110.
Vinnakota, K. C., Mitchell, D. A., Deschenes, R. J., Wakatsuki, T. & Beard, D. A. (2010). Analysis of diffusion of Ras2 in Saccharomyces cerevisiae using fluorescence recovery after photobleaching. Physical Biology 4, 026011.
Wachsmuth, M. (2014). Molecular diffusion and binding analyzed with FRAP. Protoplasma 251, 373382.
Waharte, F., Brown, C. M., Coscoy, S., Coudrier, E. & Amblard, F. (2005). A two-photon FRAP analysis of the cytoskeleton dynamics in the microvilli of intestinal cells. Biophysical Journal 88, 14671478.
Walczak, C. E., Rizk, R. S., Shaw, S. L. (2010). The use of fluorescence redistribution after photobleaching for analysis of cellular microtubule dynamics. Methods in Cell Biology 97, 3552.
Watanabe, N., Yamashiro, S., Vavylonis, D. & Kiuchi, T. (2013). Molecular viewing of actin polymerizing actions and beyond: combination analysis of single-molecule speckle microscopy with modeling, FRAP and s-FDAP (sequential fluorescence decay after photoactivation). Dev Growth Differ 55(4), 508514.
Webb, W. W. (2006). Commentary on the pleasures of solving impossible problems of experimental physiology. Annual Review of Physiology 68, 128.
Wedekind, P., Kubitscheck, U. & Peters, R. (1994). Scanning microphotolysis: a new photobleaching technique based on fast intensity modulation of a scanned laser beam and confocal imaging. Journal of Microscopy 176, 2333.
Weiss, M. 2004. Challenges and artifacts in quantitative photobleaching experiments. Traffic 5, 662671.
Wiseman, P. W., Squier, J. A., Ellisman, M. H. & Wilson, K. R. (2000). Two-photon image correlation spectroscopy and image cross-correlation spectroscopy. Journal of Microscopy 200, 1425.
Wu, E. S., Jacobson, K. & Papahadjopoulos, D. (1977). Lateral diffusion in phospholipid multibilayers measured by fluorescence recovery after photobleaching. Biochemistry 16, 39363941.
Wu, J., Shekhar, N., Lele, P. P. & Lele, T. P. (2012). FRAP analysis: accounting for bleaching during image capture. PLoS ONE 7, e42854.
Yechiel, E. & Edidin, M. (1987). Micrometer-scale domains in fibroblast plasma membranes. Journal of Cell Biology 105, 755760.
Zagato, E., Forier, K., Martens, T., Neyts, K., Demeester, J., De Smedt, S., Remaut, K. & Braeckmans, K. (2014). Single particle tracking for studying nanomaterial dynamics: applications and fundamentals in drug delivery. Nanomedicine 9, 913927.
Ziv, N. E. & Fisher-Lavie, A. (2014). Presynaptic and postsynaptic scaffolds: dynamics fast and slow. Neuroscientist 20(5), 439452.
Zuleger, N., Kelly, D. A. & Schirmer, E. C. (2013). Considering discrete protein pools when measuring the dynamics of nuclear membrane proteins. Methods in Molecular Biology 1042, 275298.

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