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Analysis of the domain structure of membranes by fragmentation and separation in aqueous polymer two-phase systems

Published online by Cambridge University Press:  17 March 2009

Per-Åke Albertsson
Per-Åke Albertsson
Affiliation:
Department of Biochemistry, University of Lund, Box 124, S 221 00 Lund, Sweden.
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Extract

This paper consists of three parts. The first describes theoretically a general strategy for fragmentation and separation of membranes which can be used in the elucidation of their structure and function. The second part describes a practical separation method, partition in liquid aqueous polymer two-phase systems, which can be used for separation of macromolecules and membrane particles of biological origin. The third part gives examples of the application of this method to membrane vesicles, and how this separation in combination with the strategy described in the first part can be used for analysis of the structure of biological membranes.

Type
Research Article
Information
Quarterly Reviews of Biophysics , Volume 21 , Issue 1 , February 1988 , pp. 61 - 98
Copyright
Copyright © Cambridge University Press 1988

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