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Neural connectivity biotypes: associations with internalizing problems throughout adolescence

  • Rajpreet Chahal (a1) (a2), David G. Weissman (a3), Michael N. Hallquist (a4), Richard W. Robins (a5), Paul D. Hastings (a2) (a5) and Amanda E. Guyer (a1) (a2)...



Neurophysiological patterns may distinguish which youth are at risk for the well-documented increase in internalizing symptoms during adolescence. Adolescents with internalizing problems exhibit altered resting-state functional connectivity (RSFC) of brain regions involved in socio-affective processing. Whether connectivity-based biotypes differentiate adolescents’ levels of internalizing problems remains unknown.


Sixty-eight adolescents (37 females) reported on their internalizing problems at ages 14, 16, and 18 years. A resting-state functional neuroimaging scan was collected at age 16. Time-series data of 15 internalizing-relevant brain regions were entered into the Subgroup-Group Iterative Multi-Model Estimation program to identify subgroups based on RSFC maps. Associations between internalizing problems and connectivity-based biotypes were tested with regression analyses.


Two connectivity-based biotypes were found: a Diffusely-connected biotype (N = 46), with long-range fronto-parietal paths, and a Hyper-connected biotype (N = 22), with paths between subcortical and medial frontal areas (e.g. affective and default-mode network regions). Higher levels of past (age 14) internalizing problems predicted a greater likelihood of belonging to the Hyper-connected biotype at age 16. The Hyper-connected biotype showed higher levels of concurrent problems (age 16) and future (age 18) internalizing problems.


Differential patterns of RSFC among socio-affective brain regions were predicted by earlier internalizing problems and predicted future internalizing problems in adolescence. Measuring connectivity-based biotypes in adolescence may offer insight into which youth face an elevated risk for internalizing disorders during this critical developmental period.


Corresponding author

Author for correspondence: Rajpreet Chahal, E-mail:; Amanda Guyer, E-mail:


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Neural connectivity biotypes: associations with internalizing problems throughout adolescence

  • Rajpreet Chahal (a1) (a2), David G. Weissman (a3), Michael N. Hallquist (a4), Richard W. Robins (a5), Paul D. Hastings (a2) (a5) and Amanda E. Guyer (a1) (a2)...


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