Skip to main content Accessibility help
×
Home

A post-translational modification of nuclear proteins, NG,NG-dimethyl-Arg, found in a natural HLA class I peptide ligand

  • JESÚS YAGÜE (a1), JESÚS VÁZQUEZ (a1) and JOSÉ A. LÓPEZ DE CASTRO (a1)

Abstract

Presentation of peptides derived from endogenous proteins by class I major histocompatibility complex molecules is essential both for immunological self-tolerance and induction of cytotoxic T-cell responses against intracellular parasites. Despite frequent and diverse post-translational modification of eukaryotic cell proteins, very few class I-bound peptides with post-translationally modified residues are known. Here we describe a natural dodecamer ligand of HLA-B39 (B*3910) derived from an RNA-binding nucleoprotein that carried NG,NG-dimethyl-Arg. Although common among RNA-binding proteins, this modification was not previously known among natural class I ligands. The sequence of this peptide was determined by Edman degradation and electrospray ion trap mass spectrometry. The fragmentation pattern of the dimethyl-Arg side chain observed with this latter technique allowed us to unambiguously assign the isomeric form of the modified residue. The post-translationally modified ligand was a prominent component (1–2%) of the B*3910-bound peptide repertoire. The dimethyl-Arg residue was located in a central position of the peptide, amenable to interacting with T-cell receptors, and most other residues in the middle region of the peptide were Gly. These structural features strongly suggest that the post-translationally modified residue may have a major influence on the antigenic properties of this natural ligand.

Copyright

Corresponding author

Reprint requests to: Dr. José A. López de Castro, Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid, Facultad de Ciencias, Cantoblanco, 28049 Madrid, Spain; e-mail: aldecastro@cbm.uam.es.

Keywords

A post-translational modification of nuclear proteins, NG,NG-dimethyl-Arg, found in a natural HLA class I peptide ligand

  • JESÚS YAGÜE (a1), JESÚS VÁZQUEZ (a1) and JOSÉ A. LÓPEZ DE CASTRO (a1)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed