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XV.—The metabolism of DNA in the liver during precocious induction of metamorphosis in Rana catesbeiana*

Published online by Cambridge University Press:  11 June 2012

Ailsa M. Campbell ,
Martita H. Corrance ,
J. N. Davidson  and
H. M. Keir
Ailsa M. Campbell
Affiliation:
Institute of Biochemistry, University of Glasgow
Martita H. Corrance
Affiliation:
Institute of Biochemistry, University of Glasgow
J. N. Davidson
Affiliation:
Institute of Biochemistry, University of Glasgow
H. M. Keir
Affiliation:
Institute of Biochemistry, University of Glasgow
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Synopsis

Studies have been made on the incorporation of [8H]-deoxythymidine into the DNA of the livers of Rana catesbeiana tadpoles. When metamorphosis was induced with tri-iodothyronine, the specific activity of the nuclear DNA rose 5 days after administration of the hormone. In contrast, the specific activity of the DNA from the mitochondrial fraction rose grave–2 days after hormone administration.

In order to determine whether the in vivo change was due to alterations in the pool sizes of the DNA precursors, in vitro studies on DNA polymerase were carried out. It was found that under conditions where the enzyme activity was not limited by availability of template or substrates, there was a rise in the DNA polymerase activity in crude cell extracts from the tadpole liver. Fractionation of the cell components showed that little of this increment in activity appeared to be located in the nucleus, but that a large percentage alteration in activity occurred in the mitochondrial and cell sap fractions.

A possible interpretation of these results is that an increase in the mitochondrial DNA polymerase is one of the early effects of thyroid hormone. This possibility is discussed in relation to the other known effects of thyroid hormones in tadpoles, with particular reference to nucleic acid metabolism and also to mitochondrial hyperplasia.

Type
Research Article
Information
Proceedings of the Royal Society of Edinburgh, Section B: Biological Sciences , Volume 70 , Issue 4 , 1969 , pp. 295 - 310
Copyright
Copyright © Royal Society of Edinburgh 1969

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Footnotes

†Department of Biochemistry, University of Aberdeen.
*

This paper was assisted in publication by a grant from the Carnegie Trust for the Universities of Scotland.

References

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