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Two steroidogenic pathways present in the granulosa layer of the preovulatory follicles of the goose

Published online by Cambridge University Press:  20 November 2017

D. Wojtysiak  and
E. Kapkowska
D. Wojtysiak
Affiliation:
Department of Animal Anatomy, University of Agriculture, 30-059 Kraków, Poland. Email, wojtysiakd@wp.pl
E. Kapkowska
Affiliation:
Department o f Poultry Breeding, University of Agriculture, 30-059 Kraków, Poland
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Extract

The follicular wall of the ovarian follicles play very important roles for the development of oocyte and ovulation. The major function of this tissue is the biosynthesis of steroids. One of the key enzyme of the steroidogenesis is 3β-hydroksysteroid dehydrogenase (3β-HSD) which converting dehydroepiandrosterone to androstenedione in the ∆5-3β-hydroksysteroid pathway and pregnenolone to progesterone in the ∆4-3-ketosteroid pathway. Lee and Bahr (1994) suggested that both ∆5 and ∆4 pathway are functional in ovarian follicles. Recently Lee et all (1998) reported that theca layer preferentially metabolises steroids via the ∆5 pathways regardless of the maturational stage of the follicles, while the granulosa layer of preovulatory follicles metabolises steroids via the ∆4 pathways. In white follicles the granulosa layer is steroidogenically inactive (Tilly et al., 1991). The changes of 3β-HSD activity in the follicles of the chiken ovary during follicular development are well known (Tilly et al., 1991). However the activity of 3β-HSD in granulosa layer of the yellow preovulatory ovarian follicles of the goose has not yet been determined. Therefore the aim of this study was to determine two steroidogenic pathways present in the isolated granulosa layer of the preovulatory follicles of the goose, by examined enzymatic activity of 3β-HSD using P5 and DEHA as a substrate.

Type
Poster Presentations
Information
Proceedings of the British Society of Animal Science , Volume 2004 , 2004 , pp. 184
Copyright
Copyright © The British Society of Animal Science 2004

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References

Gilbert, A. B., Evans, A. J., Perry, M. M., Davidson, M. H. 1977. A method for separating the granulosa cells, the basal lamina and theca of the preovulatory ovarian follicle of the domestic fowl (Gallus domesticus) . Journal of Reprodiction and Fertility., 50: 179–181.Google Scholar
Lee, K. A., Bahr, J. M. 1994. Utilization of substrates for testosterone and estradiol-17β production by small follicles of chicken ovary. Domest. Animal Endocrinology, 11: 307–314.CrossRefGoogle ScholarPubMed
Lee, K. A., Volentine, K. K., Bahr, J. M. 1998. Two steroidogenic pathways present in the chicken ovary: theca layer prefers ∆5 pathway and granulosa layer prefers ∆4 pathway. Domestic Animal Endocrinology, 15: 1–8.Google Scholar
Tilly, J. L., Kowalski, K. I., Johnson, A. L. 1991b. Stage of ovarian follicular development associated with the initiation of steroidogenic competence in avian granulosa cells. Biology Reproduction, 44: 305–314.CrossRefGoogle ScholarPubMed