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Standardization of the immunoassays (ELISA) to detection of gentamicin in livestock using polyclonal antibody

Published online by Cambridge University Press:  20 November 2017

S.P. Gobbo ,
P.A. Bricarello ,
K.M.R. Duarte ,
S.M.G. Fedrizzi ,
F.C.A. Tavares  and
C. F. Meirelles
S.P. Gobbo*
Affiliation:
Laboratory of Animal Nutrition – Centre for Nuclear Energy in Agriculture (CENA/USP), CP 96, CEP 13400-970, Piracicaba, SP, Brazil
P.A. Bricarello
Affiliation:
Laboratory of Animal Nutrition – Centre for Nuclear Energy in Agriculture (CENA/USP), CP 96, CEP 13400-970, Piracicaba, SP, Brazil
K.M.R. Duarte
Affiliation:
Laboratory of Yeast Genetics – (ESALQ/USP)
S.M.G. Fedrizzi
Affiliation:
Laboratory of Animal Nutrition – Centre for Nuclear Energy in Agriculture (CENA/USP), CP 96, CEP 13400-970, Piracicaba, SP, Brazil
F.C.A. Tavares
Affiliation:
Laboratory of Yeast Genetics – (ESALQ/USP)
C. F. Meirelles
Affiliation:
Laboratory of Animal Nutrition – Centre for Nuclear Energy in Agriculture (CENA/USP), CP 96, CEP 13400-970, Piracicaba, SP, Brazil
*
E-mail: spgobbo@cena.usp.br
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Extract

The intensification of cattle in the world and the use of veterinary substances as growing promoters in bovines, sheep, goats and poultry represent a large amount of the total cost of livestock production. Antimicrobial additives when used in animal production for long periods can accumulate residues in animal tissues, including the risk of interactions with other drugs. Global control and monitoring of the use of antibiotics in animal production are significant questions for public health due to the dissemination of microbial resistance to antibiotics drugs. In this study, the objective was to detect the gentamicin antibiotic by indirect PTA-ELISA method (plate trapped antigen. enzyme linked immunoassay), producing polyclonal antibodies from rabbits.

Type
Poster Presentations
Information
Proceedings of the British Society of Animal Science , Volume 2004 , 2004 , pp. 107
Copyright
Copyright © The British Society of Animal Science 2004

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References

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