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The use of metabolic profiles is widely accepted as an assessment method of the dairy cows’ energy status (Whitaker, 2004). Three of the most reliable parameters that can be used are serum concentration of glucose, beta hydroxybutyric acid (BHB) and non-esterified fatty acids (NEFA). There is growing interest in the changes of these parameters during lactation (Wathes et al., 2007). The scope of this study was to record and characterise the fluctuation of serum levels of glucose, BHB and NEFA during a 305-day lactation of primiparous Holstein cows raised under commercial conditions.