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Insulin stimulates phenylalanine uptake across the hind limb in fed lambs

Published online by Cambridge University Press:  20 November 2017

T.J Wester ,
G.E. Lobley ,
L.M. Birnie  and
M.A. Lomax
T.J Wester
Affiliation:
Department of Agriculture, University of Aberdeen, 581 King Street, Aberdeen AB24 5UA, Scotland
G.E. Lobley
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, Scotland
L.M. Birnie
Affiliation:
Department of Agriculture, University of Aberdeen, 581 King Street, Aberdeen AB24 5UA, Scotland
M.A. Lomax
Affiliation:
Department of Agriculture, University of Aberdeen, 581 King Street, Aberdeen AB24 5UA, Scotland
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Extract

Although insulin has been shown to stimulate muscle protein accretion in nonruminants (e.g., Wray-Cahen et al., 1998) evidence for this same effect in ruminants has been equivocal (e.g., Oddy et al., 1987; Wolff et al., 1989). Moreover, when insulin has been reported to have an anabolic effect in ruminants, the animal has been in the fasted state. In addition, Garlick and Grant (1988) have shown that branched-chain amino acids (BCAA) enhanced insulin-stimulated protein synthesis in fasted rats. The primary aim of the present study was to ascertain whether insulin increases phenylalanine (Phe) uptake (used as an index of skeletal muscle protein anabolism) across the hind limb of fed lambs. A secondary aim was to determine if infusion of BCAA, alone or in combination with insulin, would stimulate Phe uptake greater than insulin alone.

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Information
Proceedings of the British Society of Animal Science , Volume 1999 , 1999 , pp. 162
Copyright
Copyright © The British Society of Animal Science 1999

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References

Garlick, P.J. and Grant, I. 1988. Amino acid infusion increases the sensitivity of muscle protein synthesis in vivo to insulin: effect of branched-chain amino acids. Biochemical Journal 254:579–584.CrossRefGoogle ScholarPubMed
Oddy, V.H., Lindsay, D.B., Barker, P.J., and Northrop, A.J. 1987. Effect of insulin on hind-limb and whole-body leucine and protein metabolism in fed and fasted sheep. British Journal of Nutrition 55:143–154.Google Scholar
Wolff, J.E., Dobbie, P.M., and Petrie, D.R. 1989.Anabolic effects of insulin in growing lambs. Quarterly Journal of Experimental Physiology 74:451–463.CrossRefGoogle ScholarPubMed
Wray-Cahen, D., Nguyen, H.V., Burrin, D.G., Beckett, P.R., Fiorotto, M.L., Reeds, P.R., Wester, T.J., and Davis, T.A. 1998. Response of skeletal muscle protein synthesis to insulin in suckling pigs decreases with development. American Journal of Physiology (Endocrinology and Metabolism) 275:E602–E609.CrossRefGoogle ScholarPubMed