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The fatty acid composition of solid- and liquid-associated rumen bacteria isolated from cows

Published online by Cambridge University Press:  20 November 2017

S.S. Lee ,
M.B. Chang ,
N.D. Scollan ,
R.J. Merry ,
M.S. Dhanoa ,
P.J. Hobbs ,
V.J. Theobald ,
W.J. Maeng  and
R.J. Dewhurst
S.S. Lee
Affiliation:
Department of Animal Science, Chung-Ang University, Ansung 456-756, Korea
M.B. Chang
Affiliation:
Department of Animal Science, Chung-Ang University, Ansung 456-756, Korea
N.D. Scollan
Affiliation:
Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth SY23 3EB, UK
R.J. Merry
Affiliation:
Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth SY23 3EB, UK
M.S. Dhanoa
Affiliation:
Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth SY23 3EB, UK
P.J. Hobbs
Affiliation:
Institute of Grassland and Environmental Research, North Wyke, Okehampton, Devon EX20 2SB, UK
V.J. Theobald
Affiliation:
Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth SY23 3EB, UK
W.J. Maeng
Affiliation:
College of Animal Husbandry, KonKuk University, Seoul 143-701, Korea
R.J. Dewhurst
Affiliation:
Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth SY23 3EB, UK
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Extract

The distinctive (phospholipid or total) fatty acid profiles of the major classes of micro-organism have been used in microbial taxonomy as well as to act as microbial markers in soils. Our studies seek to identify the potential of this approach in rumen studies. One of the main distinctions is between solid- and liquid-associated bacteria (SAB and LAB) in the rumen, though it is not clear what relative contributions these make to microbial yield from the rumen. The objective of this work was to determine whether SAB and LAB have distinctive fatty acid profiles and if differences are affected by dietary fat supplementation.

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Programme
Information
Proceedings of the British Society of Animal Science , Volume 1999 , 1999 , pp. 30
Copyright
Copyright © The British Society of Animal Science 1999

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References

Bligh, E.G. and Dyer, W.J. 1959. A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 37:911–917.CrossRefGoogle ScholarPubMed
Merry, R.J. and McAllan, A.B. 1983. A comparison of the chemical composition of mixed bacteria harvested from the liquid and solid fractions of rumen digesta. British Journal of Nutrition 50:701–709.CrossRefGoogle ScholarPubMed