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Establishment, characterisation and mammary specific function of a bovine mammary epithelial cell clone cultured on reconstituted basement membrane

Published online by Cambridge University Press:  20 November 2017

H.R. McConochie ,
M.T. Rose ,
W.H. Haresign  and
B. Davies
H.R. McConochie
Affiliation:
Institute of Rural Sciences, Llanbadarn Campus, Llanbadarn Fawr, Ceredigion, SY23 3AL U.K. E-mail: hrm98@aber.ac.uk
M.T. Rose
Affiliation:
Institute of Rural Sciences, Llanbadarn Campus, Llanbadarn Fawr, Ceredigion, SY23 3AL U.K. E-mail: hrm98@aber.ac.uk
W.H. Haresign
Affiliation:
Institute of Rural Sciences, Llanbadarn Campus, Llanbadarn Fawr, Ceredigion, SY23 3AL U.K. E-mail: hrm98@aber.ac.uk
B. Davies
Affiliation:
Institute of Rural Sciences, Llanbadarn Campus, Llanbadarn Fawr, Ceredigion, SY23 3AL U.K. E-mail: hrm98@aber.ac.uk
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Extract

Mammary gland development and lactation are complex processes, predominantly governed by the reproductive state of the female. The many hormones, growth factors and cell types make the in vivo elucidation of the roles of these factors in mammary development and lactation difficult. Therefore, an in vitro model would be very useful. A step towards this goal was demonstrated when a mammary epithelial cell (MEC) clone was isolated by limiting dilution methodology, and morphological and functional differentiation on a reconstituted basement membrane was brought about (Rose et al. 2002). The aim of this study was to build upon these earlier observations, and establish a representative model of the bovine mammary gland using a MEC clone capable of mammary specific function.

Type
Poster Presentations
Information
Proceedings of the British Society of Animal Science , Volume 2004 , 2004 , pp. 178
Copyright
Copyright © The British Society of Animal Science 2004

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References

Barcellos-Hoff, M.H., Aggeler, J., Ram, T.G. and Bissel, M.J. 1989. Functional differentiation and alveolar morphogenesis of primary mammary cultures on reconstituted basement membrane. Development 105 223–235.CrossRefGoogle ScholarPubMed
Rose, M.T., Aso, H., Yonekura, S., Komatsu, T., Ozutsumi, K. and Obara, Y. 2002. In vitro differentiation of a cloned bovine mammary epithelial cell. Journal of Dairy Research 69 345–355.CrossRefGoogle ScholarPubMed