The concentrations of the long chain n-3 polyunsaturated fatty acids (LC n-3 PUFA) eicosapentaenoic acid (C20:5, EPA) and docosahexaenoic acid (C22:6, DHA) in poultry meat can be increased (to some extent) by enriching poultry diets with these two acids. However, this relies almost entirely on the use of fish oil as there are no terrestrial sources of these acids. This raises the issue of both the sustainability of such an approach and also the consumer acceptability of such a reliance on fish oil in poultry diets. The conversion of α-linolenic acid (LNA) to EPA and DHA, though limited, is done by birds and so an alternative approach would be to enrich poultry diets with the more abundantly available LNA as a means of increasing the EPA and DHA content of poultry meat. The objective of this study was to investigate whether there was any effect of poultry species and breed on the enrichment of poultry meat with EPA and DHA in response to increased dietary concentrations of LNA.