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Comparison of different digesta collection methods and indigestible markers to determine the apparent ileal nutrient digestibility

Published online by Cambridge University Press:  05 November 2021

Y-L Yin ,
J McEvoy ,
W-B Souffrant  and
K J McCracken
Y-L Yin
Affiliation:
Department of Agricultural and Environmental Science, The Queen's University of Belfast
J McEvoy
Affiliation:
Veterinary Science Division, Department of Agriculture, Northern Ireland
W-B Souffrant
Affiliation:
Ernahrungsphysiology “Oskar Kellner”, Forschungsinstitut fur die Biologie landwirtschaftlicher Nutztiere, Rostock
K J McCracken
Affiliation:
Department of Agricultural and Environmental Science, The Queen's University of Belfast
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Extract

Since the 1970's, many studies have been performed to estimate ileal digesibility using different methods for digesta collection. The methods frequently applied for quantitative collection are re-entrant cannulation or ileorectal anastomosis (IRA). Methods involving the use of a marker include the post- valve “T” caecal (PVTC) and simple ileal “T” cannula. Recent studies showed that PVTC has a number of advantages over the simple “T” cannulation methods (van Leeuwen et al, 1991; McCracken et al, 1996). The present study afforded the opportunity to (1) compare the PVTC with the IRA method, (2) compare the use of Cr2O3 and TiO2 as indigestible markers (3) evaluate the PVTC method for quantitative collection of digesta, using a range of diets of different fibre content.

The PVTC experiment was performed in Belfast. Twelve pigs, approximately 20kg, fitted with post valve “T” caecal cannulae according to the procedure introduced by van Leeuwen et al(1991), were randomized to 4 diets based on wheat (W, 760 g wheat/kg), wheat/middlings (WM, 380g wheat, 380 g middlings/kg), wheat/bran (WB,380 g wheat, 380 g bran/kg) or recombined wheat (WR,570 g flour, 130 g middlings, 60 g bran/kg) using a four period cross-over design.

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Information
Proceedings of the British Society of Animal Science , Volume 1997 , 1997 , pp. 68
Copyright
Copyright © British Society of Animal Science 1997

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References

McCracken, K.J., Yin, Y.-L., McEvoy, J. and Schulze, H., 1996. Effects of diet composition, feed enzymes and method of cannulation on faecal and ileal digestibility measurements in growing pigs. Animal Science, 62: 662(Abs).Google Scholar
Van Leeuwen, P, Van Kleef, D.J., Van Kempen, G.J.M., Huisman, J., and Verstegen, M.W.A., 1991. The post valve T-caecum cannulation technique; an alternative method for chyme collection in pigs. Journal of Animal Physiology and Animal Nutrition 65: 183–193.Google Scholar