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Application of a duplex PCR approach for the specific and simultaneous detection of Bifidobacterium spp. and lactobacillus spp. in duodenum, jejunum, ileum and cecum of broilers

Published online by Cambridge University Press:  22 November 2017

S Z Mirhosseini ,
A Seidavi ,
M Shivazad ,
M Chamani ,
A A Sadeghi  and
R Pourseify
S Z Mirhosseini
Affiliation:
Animal Science Department, Guilan University, Rasht, Islamic Republic of Iran Agriculture Biotechnology Research Institute, North Region of Iran, Rasht, Islamic Republic of Iran
A Seidavi*
Affiliation:
Animal Science Department, Islamic Azad University, Rasht Branch, Rasht, Islamic Republic of Iran
M Shivazad
Affiliation:
Animal Science Department, Tehran University, Tehran, Islamic Republic of Iran
M Chamani
Affiliation:
Animal Science Department, Islamic Azad University, Science and Research Branch, Tehran, Islamic Republic of Iran
A A Sadeghi
Affiliation:
Animal Science Department, Islamic Azad University, Science and Research Branch, Tehran, Islamic Republic of Iran
R Pourseify
Affiliation:
Agriculture Biotechnology Research Institute, North Region of Iran, Rasht, Islamic Republic of Iran
*
Email: alirezaseidavi@yahoo.com
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Extract

Bifidobacterium spp. and Lactobacillus spp genera are useful and important microbiota which affect poultry performance. These bacteria are natural inhabitants of the intestine of broilers. These bacteria have a unique hexose metabolism that occurs via the phosphoketolase pathway. Bifidobacterium spp. produces the vitamins B1, B2, B6, B12, nicotinic acid and folic acid. Enzymatic hydrolysis by Bifidobacterium spp. increases bioaccessibility of lipids and proteins. Lactobacillus spp. exhibit properties which include the ability to adhere to cells, persist and multiply and produce acids, hydrogen peroxide and bacteriocidal and bacteriostatic agents. The ability of Lactobacillus to inhibit bacterial pathogens including E. coli and Salmonella species and to influence parasitic infection including Cryptosporidium parvum, Eimeria acervulina, and Giardia intestinalis (Seidavi et al. 2008). The aim of this study was to develop a method for the direct and simultaneous detection of Bifidobacterium spp. and Lactobacillus spp.in the intestine by multiplex PCR.

Type
Theatre Presentations
Information
Proceedings of the British Society of Animal Science , Volume 2009 , April 2009 , pp. 207
Copyright
Copyright © The British Society of Animal Science 2009

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References

Seidavi, A.R., Mirhosseini, S.Z., Shivazad, M., Chamani, M., and Sadeghi, A.A. 2008. Journal of Rapid Methods and Automation in Microbiology. 16, 100–112.CrossRefGoogle Scholar