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Conservation of Billbergia zebrina genetic resources: AFLP polymorphism of in vitro regenerated genotypes

Published online by Cambridge University Press:  12 December 2011

Lirio L. Dal Vesco ,
Valdir M. Stefenon ,
Leocir J. Welter ,
Neusa Steiner  and
Miguel P. Guerra
Lirio L. Dal Vesco
Affiliation:
Programa de Pós-graduação em Recursos Genéticos Vegetais, Universidade Federal de Santa Catarina, 88.034-001Florianópolis, SC, Brazil
Valdir M. Stefenon
Affiliation:
Programa de Pós-graduação em Ciências Biológicas, Universidade Federal do Pampa/Campus São Gabriel, 97300-000São Gabriel, RS, Brazil
Leocir J. Welter
Affiliation:
Universidade Federal do Pampa/Campus Itaqui, 97650-000Itaqui, RS, Brazil
Neusa Steiner
Affiliation:
Programa de Pós-graduação em Recursos Genéticos Vegetais, Universidade Federal de Santa Catarina, 88.034-001Florianópolis, SC, Brazil
Miguel P. Guerra*
Affiliation:
Programa de Pós-graduação em Recursos Genéticos Vegetais, Universidade Federal de Santa Catarina, 88.034-001Florianópolis, SC, Brazil
*
*Corresponding author. E-mail: mpguerra@cca.ufsc.br
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Abstract

Biotechnological techniques comprise useful tools for the conservation of endangered plant genetic resources. In the present work, polymorphism and usefulness of amplified fragment length polymorphism (AFLP) markers in assessing the genetic diversity in populations of Billbergia zebrina were investigated in nodular cultures and adult plants of the species. AFLP markers revealed moderate-to-high genetic diversity based on the estimations of Nei's gene diversity (mean He = 0.28), Shannon index of diversity (mean HS = 0.48) and the number of polymorphic fragments (mean of 56.17 polymorphic fragments over six primer pairs). In comparison to published studies of population genetics performed in other bromeliad species, the present study suggests that natural populations of B. zebrina likely maintain high levels of genetic diversity, an important feature towards conservation of plant genetic resources. The results obtained reveal that AFLP markers comprise a powerful tool in order to assess the levels of genetic diversity in natural populations of this endangered species. Integrating AFLP markers with in vitro propagation techniques is understood as an adequate strategy for conservation programmes of this species.

Keywords

Atlantic Rain ForestBromeliaceaeBromelioideaebromeliadsepiphytegenetic diversity
Type
Short Communication
Information
Plant Genetic Resources , Volume 10 , Issue 1 , April 2012 , pp. 20 - 23
Copyright
Copyright © NIAB 2011

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