Hostname: page-component-7479d7b7d-qs9v7 Total loading time: 0 Render date: 2024-07-11T23:58:36.787Z Has data issue: false hasContentIssue false
  • English
  • Français

Quantification of β-tubulin RNA expression during asymbiotic and symbiotic development of the arbuscular mycorrhizal fungus Glomus mosseae

Published online by Cambridge University Press:  01 March 1999

BRITTA BÜTEHORN ,
VIVIENNE GIANINAZZI-PEARSON  and
PHILIPP FRANKEN
BRITTA BÜTEHORN
Affiliation:
Max-Planck-Institut für terrestrische Mikrobiologie and Laboratorium für Mikrobiologie des Fachbereichs Biologie der Philipps-Universität, Karl-von-Frisch-Straße, 35043 Marburg, Germany
VIVIENNE GIANINAZZI-PEARSON
Affiliation:
Laboratoire de Phytoparasitologie INRA/CNRS, CMSE–INRA, BV 1540, 21034 Dijon Cedex, France
PHILIPP FRANKEN
Affiliation:
Max-Planck-Institut für terrestrische Mikrobiologie and Laboratorium für Mikrobiologie des Fachbereichs Biologie der Philipps-Universität, Karl-von-Frisch-Straße, 35043 Marburg, Germany
Get access

Abstract

Experimental systems were established for the molecular analysis of different developmental stages of Glomus mosseae. RNA and DNA were simultaneously extracted from ectocarpic spores, chlamydospores, dormant and germinating sporocarps and extraradical hyphae. A β-tubulin gene fragment was obtained by PCR from spore genomic DNA and further analysed. β-tubulin RNA expression could be monitored during the fungal life cycle with a gene-specific primer by RT–PCR using an internal cRNA standard.

Type
Research Article
Information
Mycological Research , Volume 103 , Issue 3 , March 1999 , pp. 360 - 364
Copyright
© The British Mycological Society 1999

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)