Few studies have examined faeces as a substratum for nematophagous fungi, and no information is available about colonisation of faeces in tropical regions. Sheep faeces were deposited on a Brachiaria decumbens pasture in the Mata Region of Minas Gerais, Brazil. Up to 2 wk after deposition, samples were collected and cultivated on water agar (2 %) with free-living nematodes as bait. From 150 samples, 130 isolates were recovered after 3 wk. More than 70% were predators and Arthrobotrys and Monacrosporium spp. predominated. Endoparasitic species of Drechmeria, Harposporium, Myzocytium, Nematoctonus and Verticillium were also isolated.