Hostname: page-component-7479d7b7d-c9gpj Total loading time: 0 Render date: 2024-07-08T13:05:13.216Z Has data issue: false hasContentIssue false

Effects of nutritional sources on growth of one non-pathogenic strain and four strains of Fusarium oxysporum pathogenic on tomato

Published online by Cambridge University Press:  01 September 1999

C. STEINBERG
Affiliation:
INRA-CMSE, Laboratoire de Recherches sur la Flore Pathogène du Sol, 17 rue Sully-BV1540, F. 21034 Dijon Cedex, France
J. M. WHIPPS
Affiliation:
Department of Plant Pathology and Microbiology
D. A. WOOD
Affiliation:
Department of Plant Pathology and Microbiology Present address: Consultant in Microbial Technology, 6 Dunstanville Terrace, Falmouth, TR11 2SW, Cornwall, U.K.
J. FENLON
Affiliation:
Biometrics Department, Horticulture Research International, Wellesbourne, CV35 9EF, Warwick. U.K.
C. ALABOUVETTE
Affiliation:
INRA-CMSE, Laboratoire de Recherches sur la Flore Pathogène du Sol, 17 rue Sully-BV1540, F. 21034 Dijon Cedex, France
Get access

Abstract

As part of an investigation into the factors influencing the colonization of the rhizosphere and the root tissues of host plant, by pathogenic and non-pathogenic strains of Fusarium oxysporum, the effect of a range of carbon (C) sources on the growth habits of five strains of F. oxysporum were compared. The strains used were two F. oxysporum f. sp. lycopersici (Fol strains), two F. oxysporum f. sp. radicis-lycopersici (Forl strains) pathogenic on tomato and strain Fo47, a non-pathogenic strain of F. oxysporum, currently used as a biocontrol agent to reduce severity of fusarium diseases on several crops. Radial extension rates on solid media were measured using soluble saccharides (glucose, xylose and D galacturonic acid), polysaccharides (carboxymethyl cellulose, xylan and pectin) and soluble and insoluble extracts of tomato roots as C sources. Growth parameters were estimated by fitting a logistic equation to biomass data recorded from liquid culture using the same C sources.

The strains were characterized by means of the pattern of radial extension rates on the various C sources, and the two Forl strains were discriminated further from the other strains. The growth parameters were unique features of each F. oxysporum strains, whatever the C source. The pathogenic strains did not exhibit particular abilities in degrading the cell wall components. It was concluded that growth habits related to carbohydrate utilization are unique to each strain of F. oxysporum and that these traits are not related to pathogenicity.

Type
Research Article
Copyright
© The British Mycological Society 1999

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)