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Distinction between stem- and leaf-infecting forms of Melampsora rust on Salix viminalis using RAPD markers

Published online by Cambridge University Press:  01 January 1997

M. H. PEI ,
M. J. WHELAN ,
N. G. HALFORD  and
D. J. ROYLE
M. H. PEI
Affiliation:
IACR–Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol BS18 9AF, U.K.
M. J. WHELAN
Affiliation:
IACR–Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol BS18 9AF, U.K.
N. G. HALFORD
Affiliation:
IACR–Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol BS18 9AF, U.K.
D. J. ROYLE
Affiliation:
IACR–Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol BS18 9AF, U.K.
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Abstract

The RAPD technique was used to distinguish two forms of Melampsora rust on Salix viminalis. Fifteen isolates of the stem-infecting form and 19 of the leaf-infecting form collected from the U.K. were tested with 10 arbitrary primers. All the primers generated polymorphic bands and 46 putative loci were examined. Seven primers gave a total of eight bands specific for the stem-infecting form and four primers produced five bands characteristic of the leaf-infecting form. Cluster analysis using Unweighted Pair-Group Method with Averaging (UPGMA) revealed 62% disagreement between the two forms. The RAPD band patterns of the stem-infecting form isolates were almost identical, while those of the leaf-infecting isolates showed up to 29% variation.

Type
Research Article
Information
Mycological Research , Volume 101 , Issue 1 , January 1997 , pp. 7 - 10
Copyright
© The British Mycological Society 1997

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