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Species-specific PCR primers in the rDNA-ITS region as a diagnostic tool for Serpula lacrymans

Published online by Cambridge University Press:  01 January 2000

O. SCHMIDT  and
U. MORETH
O. SCHMIDT
Affiliation:
Ordinariat für Holzbiologie, Universität Hamburg, Leuschnerstrasse 91, 21031 Hamburg, Germany
U. MORETH
Affiliation:
Ordinariat für Holzbiologie, Universität Hamburg, Leuschnerstrasse 91, 21031 Hamburg, Germany
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Abstract

The ITS of the nuclear ribosomal DNA of Serpula lacrymans and S. himantioides was amplified by PCR to evaluate this DNA region as a diagnostic tool. Sequencing of the amplified ITS region revealed a size of the complete ITS of 654 bp for S. lacrymans and 650 bp for S. himantioides. The base sequence difference between the two fungi was used to design species-specific oligonucleotide PCR primers for both species. With laboratory and field samples, each primer selectively detected its target organism. No cross reaction occurred with other fungi causing rot of indoor wood.

Type
Research Article
Information
Mycological Research , Volume 104 , Issue 1 , January 2000 , pp. 69 - 72
Copyright
© The British Mycological Society 2000

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