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Biolistic transformation of Cercospora caricis, a specific pathogenic fungus of Cyperus rotundus

Published online by Cambridge University Press:  18 April 2001

Radi ALY ,
Nurit HALPERN ,
Baruch RUBIN ,
Evgenia DOR ,
Shmuel GOLAN  and
Joseph HERSHENHORN
Radi ALY
Affiliation:
Department of Weed Research, Agricultural Research Organization, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel. E-mail: radiali@netvision.net.il
Nurit HALPERN
Affiliation:
Department of Weed Research, Agricultural Research Organization, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel. E-mail: radiali@netvision.net.il
Baruch RUBIN
Affiliation:
Department of Field Crops, Vegetable and Genetics, Faculty of Agriculture, Rehovot 76100, Israel.
Evgenia DOR
Affiliation:
Department of Weed Research, Agricultural Research Organization, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel. E-mail: radiali@netvision.net.il
Shmuel GOLAN
Affiliation:
Department of Weed Research, Agricultural Research Organization, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel. E-mail: radiali@netvision.net.il
Joseph HERSHENHORN
Affiliation:
Department of Weed Research, Agricultural Research Organization, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel. E-mail: radiali@netvision.net.il
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Abstract

Cercospora caricis is being considered as a bioherbicide agent for use against purple nutsedge (Cyperus rotundus), one of the world's worst weeds. However, first its efficiency must be improved, for example by genetic transformation. By optimizing physical and biological parameters, the particle gun acceleration method (biolistics) has been adapted to transform mycelial cells of C. caricis. Two genes were expressed in C. caricis by biolistic transformation. The β-glucuronidase gene (GUS) fused to the GDP1 promoter of Cochliobolus hetrostrophus and the hygromycin B resistance gene under control of the PtrpC promoter of Aspergillus nidulans. Although the transformation frequency was not high, all transformants were stable when they were propagated on a selective medium after eight subsequent transfers.

Type
Research Article
Information
Mycological Research , Volume 105 , Issue 2 , February 2001 , pp. 150 - 152
Copyright
© The British Mycological Society 2001

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