Skip to main content Accessibility help
×
Home

X-Ray Absorption Spectroscopy of Nitrogenase Mo-Fe Protein

  • J. Chen (a1), J. Christiansen (a1), S. George (a1), R. Tittsworth (a2), B. Hales (a2), N. Campobasso (a3), J. Bolin (a3) and S. P. Cramer (a1)...

Abstract

We have studied both the atomic and electronic structures of nitrogenase Mo-Fe proteins using EXAFS and L-edge spectroscopy. The Mo and Fe K-edge EXAFS data show not only 2.37, 2.70, 2.29, and 2.63 Å first shell Mo-S, Mo-Fe, Fe-S, and Fe-Fe distances, but also 3.8, 4.3 and 5.1 Å second shell Fe-Fe, Fe-S, and Mo-Fe interactions. These observed distances are consistent with recent crystallographic models for the M-center. The average Mcenter distances have been defined with unprecedented accuracy. The nitrogenase Fe L-edge spectra have been obtained for the first time, and the result suggests that the Fe exists in both Fe2+ and Fe3+ states.

Copyright

References

Hide All
1. Orme-Johnson, W.H., Ann. Rev. Biophys. Biophys. Chem. 14, 419 (1985); F.B. Simpson and R.H. Burns, Science, 224, 1095 (1984).
2. Shah, V.K. and Brill, W.J., Proc. Natl. Acad. Sci. U.S.A. 74, 3249 (1977).
3. Kim, J. and Rees, D.C., Science, 257, 1653 (1992);
4. Kim, J. and Rees, D.C., Science, 257, 1677 (1992); Nature, 360, 553 (1992); J.T. Bolin, A.E. Ronco, T.V. Morgan, L.E. Mortenson, N.-H. Xuong, Proc. Natl. Acad. Sci. USA, 90, 1078 (1993).
5. Orme-Johnson, W.H., Science, 257, 1639 (1992); D. Sellmann, Ang. Chem. Int. Ed. Engl. 32, 54 (1993).
6. Thorneley, R.N.F. and Lowe, D.J., Biochem. J. 224, 887 (1984).
7. Conradson, S.D., Burgess, B.K., Newton, W.E., Mortenson, L.E., and Hodgson, K.O, J. Am. Chem. Soc. 109, 7507 (1987).
8. Arber, J.M., Flood, A.C., Garner, C.D., Gormal, C.A., Hasnain, S.S., and Smith, B.E., Biochem. J., 252, 421 (1988).
9. Chen, J. et. al. J. Am. Chem. Soc. (in press).
10. Rehr, J. J., Albers, R. C., Phys. Rev. B 41, 8139, (1991); J. J. Rehr, J. Mustree de Leon, S. I. Zabinsky, R. C. Albers, J. Am. Chem. Soc. 113, 5153 (1991).
11. Hales, B. J., Langosch, D. J., E. E.Case, J. Bio. Chem. 261, 15301 (1986).
12. Gurbiel, R. J., Bolin, J. T., Ronco, A. E., Mortenson, L., Hoffman, B. M., J. Magnetic Resonance, 91, 227 (1991).
13. Cramer, S. P., Tench, O., Yocum, M., George, G. N., Nucl. Inst. Meth. A 266, 586 (1988); S.P. Cramer, J. Chen, S.J. George, J. van Elp, J. Moore, O. Tensch, J. Colaresi, M. Yocum, O.C. Mullins, and C.T. Chen, Nucl. Instr. and Meth. A319, 285 (1992).
14. Flank, A. M., Weininger, M., Mortenson, L. E., Cramer, S. P., J. Am. Chem. Soc. 108, 1049 (1986).
15. Bolin, J.T., Ronco, A.E., Mortenson, L.E., Morgan, T.V., Williamson, M., and Xuong, N.-H., in Nitrogen Fixation: Achievements and Objectives, Gresshoff, Roth, Stacey, Newton, eds. Chapman and Hall, New York (1990) p. 117.
16. Chen, J. et. al. in Molybdenum Enzymes, ACS Symposium Series, Stiefel, E., ed. (in press).
17. van der Laan, G., Zaanen, J., Sawatzky, G.A., Karnatak, R., and Esteva, J.M., Phys. Rev. B, 33, 4253 (1986).
18. Groot, F.M.F. de, Fuggle, J.C., Thole, B.T., Sawatzky, G.A., Phys. Rev. B. 42,5459 (1990).
19. Thole, B.T., Cowan, R.D., Sawatzky, G.A., Fink, J., Fuggle, J.C., Phys. Rev. B. 31, 6856 (1985).
20. de Groot, F.M.F., Fuggle, J.C., Thole, B.T., Sawatzky, G.A., Phys. Rev. B. 41, 928 (1990).
21. George, S.J., van Elp, J., Chen, J., Ma, Y., Chen, C.T., Park, J.B., Adams, M.W.W., Searle, B.G., de Groot, F.M.F., Fuggle, J.C., and Cramer, S.P., Amer. Chem. Soc., 114, 442 (1992).
22. Chen, C.T. and Sette, F., Rev. Sci. Instr. 60, 1616 (1989); C.T. Chen, F. Sette, Y. Ma, S. Modesti, Phys. Rev. B, 42, 7262 (1990).
23. Elp, J. van et. al. submitted to Proc. Natl. Acad. Sci.
24. Workshop Report, LBL-31854, 1991.

X-Ray Absorption Spectroscopy of Nitrogenase Mo-Fe Protein

  • J. Chen (a1), J. Christiansen (a1), S. George (a1), R. Tittsworth (a2), B. Hales (a2), N. Campobasso (a3), J. Bolin (a3) and S. P. Cramer (a1)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed