Hostname: page-component-848d4c4894-pjpqr Total loading time: 0 Render date: 2024-06-20T13:30:07.749Z Has data issue: false hasContentIssue false
  • English
  • Français

Synthesis of Sol-Gel Matrices for Encapsulation of Enzymes Using an Aqueous Route

Published online by Cambridge University Press:  10 February 2011

R.B. Bhatia ,
C.J. Brinker ,
C.S. Ashley  and
T.M. Harris
R.B. Bhatia
Affiliation:
University of New Mexico, Albuquerque, NM 87106
C.J. Brinker
Affiliation:
Sandia National Laboratories, Albuquerque, NM 87185
C.S. Ashley
Affiliation:
Sandia National Laboratories, Albuquerque, NM 87185
T.M. Harris
Affiliation:
University of Tulsa, Tulsa, OK 74104.
Get access

Abstract

Sol-gel matrices are promising host materials for potential chemical and biosensor applications. Previous studies have focused on modified sol-gel routes using alkoxides for encapsulation of enzymes. However the formation of alcohol as a byproduct during hydrolysis and condensation reactions poses limitations. We report the immobilization of glucose oxidase and peroxidase in silica prepared by an aqueous route which may provide a more favorable environment for the biomolecules. A two step aqueous sol-gel procedure using sodium silicate as the precursor was developed to encapsulate the enzymes and the dye precursor, o-dianisidine. Glucose oxidase catalyzes the oxidation of glucose to give gluconic acid and hydrogen peroxide. Peroxidase then catalyzes the reaction of the dye precursor with hydrogen peroxide to produce a colored product. The kinetics of the coupled enzymatic reactions were monitored by optical spectroscopy and compared to those occurring in tetramethyl orthosilicate (TMOS) derived silica matrices developed by Yamanaka et al. [1]. Enhanced kinetics in the aqueous silicate matrices were related to differences in the host microstructures as elucidated by microstructural comparisons of the corresponding aerogels.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 519: Symposium O – Organic/Inorganic Hybrid Materials , 1998 , 183
Copyright
Copyright © Materials Research Society 1998

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1 Yamanaka, S.A., Nishida, F., Ellerby, L.M., Nishida, C.R., Dunn, B.S., Valentine, J.S. and Zink, J.I., Chemistry of Materials, 4 (3), (495), (1992).10.1021/cm00021a001Google Scholar
2 Lev, O., Tsionsky, M., Rabinovich, L., Glezer, V., Sampath, S., Pankratov, I., and Gun, J., Analytical Chemistry, 67, A22, (1995).10.1021/ac00097a711Google Scholar
3 Lin, J. and Brown, C.W., Trends in Analytical Chemistry, 16 (4), (200), (1997).10.1016/S0165-9936(97)00021-6Google Scholar
4 Ingersoll, C.M. and Bright, F.V., Chemtech, 27 (1), (26), (1997).Google Scholar
5 Brinker, C.J., Scherer, G. in Sol-Gel Science: The Physics and Chemistry of Sol-Gel Processing, Academic Press, San Diego, (1990).Google Scholar
6 Ellerby, L.M., Nishida, C.R., Nishida, F., Yamanaka, S.A., Dunn, B.S., Valentine, J.S. and Zink, J.F., Science, 255, 1113, (1992).10.1126/science.1312257Google Scholar
7 Iler, R.K. in The Chemistry of Silica: Solubility, Polymerization, Colloid and Surface Properties, and Biochemistry (John Wiley, New York, 1989), pp. 172–220.Google Scholar
8 Okkerse, K. in Physical and Chemical Aspects of Adsorbents and Catalysts, edited by Linsen, B.G. (Academic Press, London, 1970), pp. 215–228.Google Scholar
9 Bergmeyer, H.U. in Methods of enzymatic analysis, (Academic Press, New York, 1965) pp. 123–124.Google Scholar
10 Tewari, P.H., Hunt, A.J. and Lofftus, K.D., Mater. Lett., 3, 363, (1985).10.1016/0167-577X(85)90077-1Google Scholar
11 Dave, B.C., Dunn, B., J.S Valentine and Zink, J.I., ACS Symp. Series, 622, 351, (1996).10.1021/bk-1996-0622.ch024Google Scholar