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Synthesis and Characterization of Polymer Substrates for Rat H Epatocyte Culture

Published online by Cambridge University Press:  15 February 2011

Annie Tang Gutsche ,
Joanne Zurlo ,
Hungnan Lo  and
Kam W. Leong
Annie Tang Gutsche
Affiliation:
Departments of Biomedical Engineering, The Johns Hopkins University, Baltimore, MD 21205.
Joanne Zurlo
Affiliation:
Environmental Health Sciences The Johns Hopkins University, Baltimore, MD 21205.
Hungnan Lo
Affiliation:
Chemical Engineering, The Johns Hopkins University, Baltimore, MD 21205.
Kam W. Leong
Affiliation:
Departments of Biomedical Engineering, The Johns Hopkins University, Baltimore, MD 21205.
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Abstract

Lactose and heparin were covalently coupled to poly(chloromethyl styrene) and the modified polymer was used as a substrate for rat hepatocyte culture. Lactose and heparin are recognized by rat hepatocytes and can be used to mediate cell attachment to the substrate. Rat hepatocytes cultured in serum-free media on these substrates were able to maintain enzymes and peptides important in the detoxification functions of hepatocytes, without the addition of hormones such as dexamethasone, media additives such as DMSO, or complex biological extracellular factors.

The growing interest in large-scale cell culture and in tissue engineering requires substrates of different geometries. Therefore, we have fabricated the derivatized polymers into microcarriers and, most recently, foams. These three-dimensional structures, combined with the chemistries of the polymers, provided the hepatocytes with more cell-cell interactions and in vivo-like geometries than conventional flat-dish culture.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 330: Symposium S – Biomolecular Materials by Design , 1993 , 243
Copyright
Copyright © Materials Research Society 1994

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References

REFERENCES

[1] Cima, L. G., etal., J. ofBiomcchanical Engin. 113, 143 (1991).Google Scholar
[2] Ben-Ze'ev, A., Robinson, G.S., Bucher, N. L. R., Farmer, S. R., Proc. Natl. Acad. Sci. USA 85, 2161 (1988).Google Scholar
[3] Folkman, J. andMoscona, A., Nature 243, 345 (1978).Google Scholar
[4] Kobayashi, A., Kobayashi, K., Akaike, T., J. Biomater. Sci. Polymer Edn. 3 (6), 499–508 (1991).Google Scholar
[5] Akaike, T., Kobayashi, A., Kobayashi, K., Sumitomo, H., J. Bioactive Compatible Polym. 4, 51–56 (1989).Google Scholar
[6] Fujita, M., et al., Hepatology 7 (1), 1S–9S (1987).Google Scholar
[7] Cahn, F., Trends in Biotechnology 8, 131–136, (1990).Google Scholar
[8] Mikos, A. G., Sarakinos, G., Leite, S. M., Vacanti, J. P., Langer, R., Biomaterials, 14 (5) (1993)Google Scholar
[9] Lo, H., Kadiyala, S., Guggino, S., Leong, K. W., presented at the 1993 MRS Fall Meeting, Boston, MA.Google Scholar
[10] Seglen, P.O., Methods Cell Biol. 13, 29 (1976).Google Scholar
[11] Bell, E., Ivarsson, B., Merrill, C., Proc. Natl. Acad. Sci., U.S.A., 76, 1274–1278 (1979).Google Scholar
[12] Reiners, J. J., et al., Anal. Biochem. 188 (2), 317–324 (1990).Google Scholar
[13] Hissin, P. J. and Hilf, R., Anal. Biochem, 74, 214–266 (1976).Google Scholar