Hostname: page-component-8448b6f56d-mp689 Total loading time: 0 Render date: 2024-04-24T10:46:44.068Z Has data issue: false hasContentIssue false
  • English
  • Français

Surface Plasmon Resonance Spectroscopic Study on Pore-Forming Behavior of Streptolysin O on Supported Phospholipid Bilayers

Published online by Cambridge University Press:  15 February 2011

Thomas Wilkop ,
Danke Xu  and
Quan Cheng
Thomas Wilkop
Affiliation:
Department of ChemistryUniversity of CaliforniaRiverside, California 92521
Danke Xu
Affiliation:
Department of ChemistryUniversity of CaliforniaRiverside, California 92521
Quan Cheng
Affiliation:
Department of ChemistryUniversity of CaliforniaRiverside, California 92521
Get access

Abstract

A novel supported bilayer membrane was formed on top of a hydrophobic spacer layer of hexyl thioctate (HT) and its application in the detection of pore forming toxins was demonstrated. The formation kinetics, effect of different flow rates on the fusion process of vesicles and perforation by incubation with streptolysin O (SLO) and pristine membranes were investigated. Individual layers of the multilayer structure were successfully characterized with surface plasmon resonance (SPR), and the resulting spectra were analyzed in order to determine the layer thickness and other membrane properties. The interaction of SLO with the pristine biomimetic membrane resulted in a pronounced change in the reflectivity spectrum. Elements of these spectral changes were analyzed to explain the process of toxin binding and pore formation taking place on the lipid membrane.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 774: Symposium O – Materials Inspired by Biology , 2003 , O7.19
Copyright
Copyright © Materials Research Society 2003

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

(1) van der Goot, F. G.; Editor Pore-Forming Toxins. [In: Curr. Tip. Microbiol. Immunol., 2001; 257], 2001.Google Scholar
(2) Bhakdi, S.; Bayley, H.; Valeva, A.; Walev, I.; Walker, B.; Weller, U.; Kehoe, M.; Palmer, M. Archives of Microbiology 1996, 165, 73–79.Google Scholar
(3) Alouf, J. E.; Freer, J. H.; Editors Comprehensive Sourcebook of Bacterial Protein Toxins, second Edition, 1999.Google Scholar
(4) Sekiya, K.; Danbara, H.; Yase, K.; Futaesaku, Y. Journal of Bacteriology 1996, 178, 6998–7002.Google Scholar
(5) Glazier, S. A.; Vanderah, D. J.; Plant, A. L.; Bayley, H.; Valincius, G.; Kasianowicz, J. J. Langmuir 2000, 16, 10428–10435.Google Scholar
(6) Song, X.; Nolan, J.; Swanson, B. Journal of the American Chemical Society 1998, 120, 4873–4874.Google Scholar
(7) Hubbard, J. B.; Silin, V.; Plant, A. L. Biophysical Chemistry 1998, 75, 163–176.Google Scholar
(8) Pockrand, I. Surf. Sci. 1978, 72, 577–588.Google Scholar
(9) Salamon, Z.; Macleod, H. A.; Tollin, G. Biochimica et Biophysica Acta 1997, 1331, 117–129.Google Scholar
(10) Homola, J.; Yee, S. S.; Gauglitz, G. Sensors and Actuators, B: Chemical 1999, B54, 3–15.Google Scholar
(11) Salamon, Z.; Macleod, H. A.; Tollin, G. BIOCHIMICA ET BIOPHYSICA ACTA FIELD Publication Date:1997 Sep 8, 1331, 131–152. FIELD Reference Number:144 FIELD Journal Code:0217513 FIELD Call Number:.Google Scholar
(12) Mozsolits, H.; Thomas, W. G.; Aguilar, M.-I. Journal of Peptide Science 2003, 9, 77–89.Google Scholar
(13) Terrettaz, S.; Stora, T.; Duschl, C.; Vogel, H. Langmuir 1993, 9, 1361–1369.Google Scholar
(14) Azzam, R.M. A.; Bashara, N. M. Ellipsometry and Polarized Light, 1977.Google Scholar
(15) Liebermann, T.; Knoll, W. Colloids and Surfaces, A: Physicochemical and Engineering Aspects 2000, 171, 115–130.Google Scholar
(16) Lingler, S.; Rubinstein, I.; Knoll, W.; Offenhaeusser, A. Langmuir 1997, 13, 7085–7091.Google Scholar
(17) Kotsev, S. N.; Dushkin, C. D.; Ilev, I. K.; Nagayama, K. Colloid and Polymer Science 2003, 281, 343–352.Google Scholar