Skip to main content Accessibility help
×
Home

Surface Enhanced Vibrational Spectroscopy of Proteins with Plasmonic Nanoantenna Arrays

  • Ronen Adato (a1) (a2), Ahmet A. Yanik (a1) (a2), Jason J. Amsden (a3), David Kaplan (a3), Fiorenzo Omenetto (a3) (a4), Mi K. Hong (a5) (a2), Shyamsunder Erramilli (a5) (a6) (a2) and Hatice Altug (a1) (a7) (a2)...

Abstract

Infrared absorption spectroscopy is a powerful tool for structural and functional studies of biomolecules. The technique enables direct access to the vibrational fingerprints of molecular bonds in the mid-infrared spectral region (3-20μm). Although intrinsic absorption cross-sections are nearly ten orders of magnitude greater than corresponding Raman cross-sections, they are still small in comparison with those of fluorescent molecules. Sensitivity improvements are therefore required for the method to be applicable to single molecule / molecular layer studies. In this work, we demonstrate the use of lithographically patterned arrays of nanoantennas to enhance the absorption signature of the protein amide-I and II backbone vibrations. Strong absorption signals from monolayer thickness films are obtained. By arranging ensembles of tailored antennas in specific lattices, higher quality factor resonances and increased near-field intensities are possible. These features are leveraged to obtain 104-105 fold signal enhancements and the direct measurement of vibrational spectra of proteins at zepto-mole sensitivity levels.

Copyright

Corresponding author

*Corresponding author, altug@bu.edu

References

Hide All
1. Lal, S., Link, S. and Halas, N. J., Nature Photon. 1, 641 (2007).
2. Kneipp, K., Wang, Y., Kneipp, H., Perelman, L. T., Itzkan, I., Dasari, R. R. and Feld, M. S., Phys. Rev. Lett. 78, 1667 (1997).
3. Osawa, M. and Ikeda, M., J. Phys. Chem. 95, 9914 (1991).
4. Neubrechh, F., Pucci, A., Cornelius, T. W., Karim, S., Garcia-Extarri, A. and Aizpurua, J., Phys. Rev. Lett. 101, 157403 (2008)
5. Adato, R., Yanik, A. A., Amsden, J. J., Kaplan, D., Omenetto, F., Hong, M. K., Erramilli, S. and Altug, H., Proc. Nat. Acad. Sci. USA, 106, 19227 (2009).
6. Adato, R., Yanik, A. A., Wu, C.-H., Shvets, G. and Altug, H., Opt. Express 18, 4526 (2010).
7. Zou, S., Janel, N. and Schatz, G. C., J. Chem. Phys. 120, 10871 (2004).
8. Lawrence, B. D., Omenetto, F. G., Chi, K. and Kaplan, D. L., J. Mater. Sci. 43, 6967 (2008).
9. Novotny, L., Phys. Rev. Lett., 98, 266802 (2007).

Keywords

Surface Enhanced Vibrational Spectroscopy of Proteins with Plasmonic Nanoantenna Arrays

  • Ronen Adato (a1) (a2), Ahmet A. Yanik (a1) (a2), Jason J. Amsden (a3), David Kaplan (a3), Fiorenzo Omenetto (a3) (a4), Mi K. Hong (a5) (a2), Shyamsunder Erramilli (a5) (a6) (a2) and Hatice Altug (a1) (a7) (a2)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed