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Surface Bio-functionalization of boron doped diamond

  • Mathias Bonnauron (a1), Charles Agnes (a2), Pascal Mailley (a3), Jean-Charles Arnault (a4), Jacques de Sanoit (a5), Christine Mer (a6), Licinio Rocha (a7), Philippe Bergonzo (a8), Milos Nesladek (a9), Frank Omnes (a10) and Omar Elmazria (a11)...

Abstract

Biotin was grafted onto boron doped nanocrystaline diamond electrode through a four-step-chemical process. First step of grafting was electro-chemical reduction of p-4,nitro diazonium salts. The grafting was characterized using X-ray Photoelectron Spectroscopy (XPS), Atomic Force Microscopy (AFM) and fluorescence analysis. XPS analysis and curve fitting procedure lead to the identification of different bond states of carbon present in the grafted film and highlights difference of coverage rate between samples. AFM highlights the non-mononuclear aspect of the diazonium electrografting. Fluorescence measurements with avidin recognition prove the efficient of the grafting.

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References

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[1] Sanoit, J De, Bonnauron, M., Arnault, JC, Christine Mer, , Nesladek, M., Bergonzo, P., to be published in Journal of Electrochemistry Society.
[2] Wang, Jian, Carlisle, John A., Diam. Relat. Mater. 15, 279 (2006).10.1016/j.diamond.2005.09.017
[3] “High resolution XPS of organic polymers Handbook”, Beamson, G. and Briggs, D., John Wiley and Sons Ltd, Chichester, England (1992).
[4] Wang, Y., Hoffman, R.W. and Angus, J.C., J. Vac.Sci.Technol. A 2226 (1990)10.1116/1.576740
[5] Katiyar, M., Yang, Y.H., Abelson, J.R., J. Appl. Phys. 78 1659 (1995).
[6] Ballutaud, D., Simon, N., Girard, H., Rzepka, E., Bouchet-Fabre, B., Diam. Relat. Mater. 15, 716 (2006).10.1016/j.diamond.2006.01.004

Keywords

Surface Bio-functionalization of boron doped diamond

  • Mathias Bonnauron (a1), Charles Agnes (a2), Pascal Mailley (a3), Jean-Charles Arnault (a4), Jacques de Sanoit (a5), Christine Mer (a6), Licinio Rocha (a7), Philippe Bergonzo (a8), Milos Nesladek (a9), Frank Omnes (a10) and Omar Elmazria (a11)...

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