Hostname: page-component-5c6d5d7d68-txr5j Total loading time: 0 Render date: 2024-08-08T05:34:05.252Z Has data issue: false hasContentIssue false
  • English
  • Français

Silicon Microchannel Array as a Basis of Biosensor Device

Published online by Cambridge University Press:  01 February 2011

Alexander Lomzov ,
Natalia Vandysheva ,
Alexander Bublikov ,
Dmitry Pyshnyi  and
Sergei Romanov
Alexander Lomzov
Affiliation:
lomzov_alex@gorodok.netInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of RASLavrentiev Ave.8Novosibirsk n/a 630090Russian Federation
Natalia Vandysheva
Affiliation:
vandysheva@isp.nsc.ru, Institute of Semiconductor Physics, Siberian Branch of RAS, Lavrentiev Ave.13, Novosibirsk, N/A, 630090, Russian Federation
Alexander Bublikov
Affiliation:
bublikov@isp.nsc.ru, Institute of Semiconductor Physics, Siberian Branch of RAS, Lavrentiev Ave.13, Novosibirsk, n/a, 630090, Russian Federation
Dmitry Pyshnyi
Affiliation:
pyshnyi@niboch.nsc.ru, Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of RAS, Lavrentiev Ave.8, Novosibirsk, n/a, 630090, Russian Federation
Sergei Romanov
Affiliation:
romanov@isp.nsc.ru, Institute of Semiconductor Physics, Siberian Branch of RAS, Lavrentiev Ave.13, Novosibirsk, n/a, 630090, Russian Federation
Get access

Abstract

This work shows the possibilities of using the silicon-based microchannel array (Si-MCA) as a 3D-solid support of DNA probes and studying of application of optical methods for DNA hybridization direct analysis. It was obtained that amount of immobilized oligodeoxyribonucleotides on the Si-MCA surface is enhanced with lighting of Si-MCA microchannel. The sample of 160 μm thickness succeeded in immobilizing oligonucleotides 40 times as much as the standard glass slides having the same external surface. The application of Si-MCA as a carrier of DNA allows doing direct analysis of nucleic acids in infrared region by the FTIR method that essentially simplifies creation of a biosensor based on the Si-MCA. It is indicated that using of the Si-MCA as a diffraction grating in visible and UV spectra allows simplifying the spectral analysis of biochemical reaction. It was success confidently to distinguish the mediums differing on the refractive index by dn=0.006.

Keywords

sensoroptical propertiesbiomedical
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 915: Symposium R – Nanostructured Materials and Hybrid Composites for Gas Sensors and Biomedical Applications , 2006 , 0915-R03-06
Copyright
Copyright © Materials Research Society 2006

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1 Drmanac, R., Labat, I., Brukner, I., Crkvenjakov, R., Genomics 4, 114–128 (1989).Google Scholar
2 Mir, K. U., Southern, E. M., Nat. Biotechnol. 17, 788–792 (1999).Google Scholar
3 Cheek, B. J., Steel, A. B., Torres, M., Yu, Y.-Y., Yang, H. and Cooper, J., Anal. Chem. 73, 5777–5783 (2001).Google Scholar
4 Benoit, V., Steel, A., Torres, M., Yu, Y.-Y. and Yang, H., Anal. Chem. 73, 2412–2420 (2001).Google Scholar
5 Fredrickson, H. L., Fredrickson, H. L., Perkins, E. J., Bridges, T. S., Tonucci, R. J., Fleming, J. K., Nagel, A., Diedrich, K., Mendez-Tenorio, A., Doktycz, M. J., Beattie, K. L., Sci Total Environ. 274, 137–149 (2001).Google Scholar
6 Matsumoto, F., Nishioa, K. and Masuda, H., The Electrochemical Society Inc. 205th Meeting, Abs. 745 (2004).Google Scholar
7 Ouameur, A. A. and Tajmir-Riahi, H.-A., J. Biol. Chem. 279, 42041–42054 (2004).Google Scholar
8 Alex, S. and Dupuis, P., Inorganica Chimica Acta 157, 271–281 (1989).Google Scholar
9 Dovbeshko, G.I., Gridina, N. Y., Kruglova, E. B., Pashchuk, O. P., Talanta 53, 233–246 (2000).Google Scholar