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Rapid Diagnosis of E. Coli using Carbon Nanotube Field Effect Transistor Direct Binding Assay

  • Woo Jae Park (a1), Sung-Jae Chung (a2) (a3), Man S. Kim (a3), Steingrimur Stefansson (a3) and Saeyoung Ahn (a3)...

Abstract

Enzyme-Linked Immuno-Sorbent Assay (ELISA), and other methods based on the same principle, are sensitive and specific, but they suffer from several disadvantages, such as their inherent complexity and requirement for multiple reagents, incubation and washing steps and require a relatively large sample size. We have adapted a new carbon nanotube field effect transistors (CNT-FET) based platform to capture Escherichia coli antigens using only the capture anti-body showing good correlation with an established ELISA assay contrived positive and negative specimens were used to test the new CNT-FET platform and results were obtained within three minutes per each sample. The test is easy to perform, rapid, and cost efficient making it a valuable screening tool for E. coli. In this study, we looked at the applicability of using CNT field effect transistor based biosensor as a rapid diagnostic platform for Escherichia coli O157:H7. The CNT-FETs platform detected positive E. coli samples in three minutes using only 2.5 μL of sample volume. This low sample volume required by the CNT-FET platform can be especially advantageous for diagnostic tests constricted by limited amount of samples.

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1. Luppa, P.B., Sokoll, L.J. and Chan, D.W., Immunosensors: principles and applications to clinical chemistry. Clin. Chim. Acta, 314 (2001), pp. 126.
2. Wells, M., Advances in optical detection strategies for reporter signal measurements. Curr. Opin. Biotechnol., 17 (2006), pp. 2833.
3. Seydack, M., Nanoparticle labels in immunosensing using optical detection methods. Biosens. Bioelectron., 20 (2005), pp. 24542469.
4. Wang, Y., Tang, Z. and Kotov, N.A., Bioapplication of nanosemiconductors. Mater. Today, 8 (2005), pp. 2031.
5. Gruner, G., Carbon nanotube transistors for biosensing applications. Anal. Bioanal. Chem., 384 (2006), pp. 322335.
6. Star, A., Gabriel, J.C.P., Bradley, K. and Grulner, G., Electronic detection of specific protein binding using nanotube FET devices. Nano Lett., 3 (2003), pp. 459463.
7. Drummond, T. G., Hill, M. G., Barton, J. K., Nature Biotechnol. 21(2003) pp. 11921199.
8. Bange, A., Halsall, H. B., Heineman, W. R., Biosens. Bioelectron. 20(2005), pp.24882503.
9. Lee, M., Im, J., Lee, B. Y., Myung, S., Kang, J., Huang, L., Kwon, Y. K., Hong, S., Nat. Nanotechnol. 1 (2006b), pp. 6671.
10. Kim, J. P., Lee, B. Y., Lee, J., Hong, S., Sim, S. J., Biosens. Bioelectron. 24 (2009), pp. 3723378.
11. Chen, R.J., Zhang, Y., Wang, D. and Dai, H., Noncovalent sidewall functionalization of single-walled carbon nanotubes for protein immobilization. J. Am. Chem. Soc., 123 (2001), pp. 38383839.
12. Dresselhaus, M. S., Eklund, P. C., 1996. Science of Fullerenes and Carbon Nanotubes: Their Properties and Applications. Academic Press, New York.
13. Drummond, T. G., Hill, M. G., Barton, J. K., Nature Biotechnol. 21 (2003), pp.11921199.
14. Kim, J. P., Lee, B. Y., Lee, J., Hong, S., Sim, S. J., Analytical Biochemistry, 381(2) (2008) pp. 193198.

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