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Preparation and Electrochemical Characterization of DNA-modified Nanocrystalline Diamond Films

Published online by Cambridge University Press:  11 February 2011

Wensha Yang ,
Orlando Auciello ,
James E. Butler ,
Wei Cai ,
John A. Carlisle ,
Jennifer E. Gerbi ,
Dieter M. Gruen ,
Tanya Knickerbocker ,
Tami L. Lasseter  and
John N. Russell Jr
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Wensha Yang
Affiliation:
Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue Madison, WI 53706
Orlando Auciello
Affiliation:
Materials Science Division Argonne National Laboratory 9700 S. Cass Ave. Argonne, Illinois 60439
James E. Butler
Affiliation:
Naval Research Laboratory 4555 Overlook Ave. S.W. Washington, DC 20375
Wei Cai
Affiliation:
Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue Madison, WI 53706
John A. Carlisle
Affiliation:
Materials Science Division Argonne National Laboratory 9700 S. Cass Ave. Argonne, Illinois 60439
Jennifer E. Gerbi
Affiliation:
Materials Science Division Argonne National Laboratory 9700 S. Cass Ave. Argonne, Illinois 60439
Dieter M. Gruen
Affiliation:
Materials Science Division Argonne National Laboratory 9700 S. Cass Ave. Argonne, Illinois 60439
Tanya Knickerbocker
Affiliation:
Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue Madison, WI 53706
Tami L. Lasseter
Affiliation:
Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue Madison, WI 53706
John N. Russell Jr
Affiliation:
Naval Research Laboratory 4555 Overlook Ave. S.W. Washington, DC 20375
Lloyd M. Smith
Affiliation:
Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue Madison, WI 53706
Robert J. Hamers
Affiliation:
Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue Madison, WI 53706
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Abstract

Nanocrystalline diamond thin films of sub-micron thickness have been covalently modified with DNA oligonucleotides. Quantitative studies of hybridization of surface-bound oligonucleotides with fluorescently tagged complementary and non-complementary oligonucleotides were performed. The results show no detectable nonspecific adsorption, with extremely good selectivity between matched and mismatched sequences. Impedance spectroscopy measurements were made of DNA-modified boron-doped nanocrystalline diamond films. The results show that exposure to non-complementary sequences induce only small changes in impedance, while complementary DNA sequences produce a pronounced decrease in impedance. The combination of high stability, selectivity, and the ability to directly detect DNA hybridization via electrical means suggest that diamond may be an ideal substrate for continuously-monitoring biological sensors.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 737 , 2002 , F4.4
Copyright
Copyright © Materials Research Society 2003

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