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Polyethyleneimine Functionalized ZnO Quantum Dots and their Binding Interaction with Bovine Serum Albumin Protein

  • Prachi Joshi (a1) (a2), Soumyananda Chakraborti (a3), Pinak Chakrabarti (a3), Surinder P. Singh (a1) (a4), Z. A. Ansari (a2) and Virendra Shanker (a1)...

Abstract

Water stable fluorescent ZnO quantum dots (QDs) have been synthesized by wet chemical route in presence of hydrophilic capping agent polyethyleneimine (PEI) as stabilizing agent. The binding interaction of prepared ZnO QDs is studied with bovine serum albumin (BSA) protein. X-ray diffraction measurement reveals hexagonal wurtzite structure of as synthesized ZnO QDs with an average size 4-6 nm, determined using Scherer’s equation and confirmed by transmission electron microscopy (TEM). The ZnO/PEI QDs exhibit strong yellow-green emission centered at 555 nm (2.23 eV). The interaction between BSA and ZnO/PEI QDs has been studied by using spectroscopic and calorimetric methods. Static mode of tryptophan quenching in BSA by ZnO/PEI QDs indicates that a ground state complex formation is taking place between ZnO/PEI and BSA, where, the week interactions (hydrogen bonding and hydrophobic interaction) are contributing towards the stability of the complex.

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*Address for correspondence: pinak@boseinst.ernet.in; surinder.singh@upr.edu

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Both authors have equal contribution

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1. Bruchez, M. Jr., Moronne, M., Gin, P., Weiss, S. and Alivisatos, A. P., Science 281, 20132016 (1998).
2. Larson, D. R., Zipfel, W. R., Williams, R. M., Clark, S. W., Bruchez, M. P., Wise, F. W. and Webb, W. W., Science 300, 14341436 (2003).
3. Wang, X., Kong, X., Yu, Y. and Zhang, H., J. Phys. Chem. C 111, 38363841 (2007).
4. Zhou, J., Xu, N. and Wang, Z. L., Adv. Mater. 18, 24322435 (2006).
5. Hanley, C., Layne, J., Punnoose, A., Reddy, K. M., Coombs, I., Coombs, A., Feris, K. and Wingett, D., Nanotechnology 19, 295103 (2008).
6. Moussodia, R. O., Balan, L. and Schneider, R., New J. Chem. 32, 13881393 (2008).
7. Wiseman, J. W, Goddard, C. A, McLelland, D. and H Colledge, W., Gene Ther. 10, 16541662 (2003).
8. Carrabino, S., Gioia, S. D., Copreni, E., Conese, E., J. Gene Med. 7, 15551564 (2005).
9. Jisha, V.S., Arun, K.T., Hariharan, M. and Ramaiah, D., J. Am. Chem. Soc. 128, 60246025 (2006).
10. Peters, T. Jr., Adv. Protein Chem. 37, 6006560065 (1985).
11. Joshi, P., Ansari, Z. A., Singh, S. P. and Shanker, V., Adv. Sci. Lett. 2, 360363 (2009).
12. Yadav, H. K., Sreenivas, K., Gupta, V., Singh, S. P., and Katiyar, R. S., J. Mater. Res. 22, 24042409 (2007).
13. Lakowicz, J.R., Principles of Fluorescence Spectroscopy. 3rd ed.: Springer: New York, 2006.
14. Ware, W.R., J. Phys. Chem. 66, 455458 (1962).
15. Ross, P.D. and Subramanian, S., Biochemistry 20, 30963102 (1981).

Keywords

Polyethyleneimine Functionalized ZnO Quantum Dots and their Binding Interaction with Bovine Serum Albumin Protein

  • Prachi Joshi (a1) (a2), Soumyananda Chakraborti (a3), Pinak Chakrabarti (a3), Surinder P. Singh (a1) (a4), Z. A. Ansari (a2) and Virendra Shanker (a1)...

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