Skip to main content Accessibility help
×
Home

Novel three Dimensional Biodegradable Scaffolds for Bone Tissue Engineering

  • Kacey G. Marra (a1), Phil G. Campbell (a1), Paul A. Dimilla (a2), Prashant N. Kumta (a1) (a3), Mark P. Mooney (a4), Jeffrey W. Szem (a5) and Lee E. Weiss (a1) (a6)...

Abstract

We have constructed osteogenic scaffolds using solid freeform fabrication techniques. Blends of biodegradable polymers, polycaprolactone and poly(D,L-lactic-co-glycolic acid), have been examined as scaffolds for applications in bone tissue engineering. Hydroxyapatite granules were incorporated into the blends and porous discs were prepared. Mechanical properties and degradation rates of the composites were determined. The discs were seeded with rabbit bone marrow or cultured bone marrow stromal cells and in vitro studies were conducted. Electron microscopy and histological analysis revealed an osteogenic composite that supports bone cell growth not only on the surface but throughout the 1 mm thick scaffold as well. Seeded and unseeded discs were mechanically assembled in layers and implanted in a rabbit rectus abdominis muscle. Bone growth was evident after eight weeks in vivo. Electron microscopy and histological analyses indicate vascularization and primitive bone formation throughout the seeded composite, and also a “fusion” of the layers to form a single, solid construct. Finally, we have begun to incorporate the growth factor IGF-I into the scaffold to enhance osteogenicity and/or as an alternative to cell seeding.

Copyright

References

Hide All
1.) Ishuag, S. L.; Yaszemski, M. J.; Bizios, R.; Mikos, A. G., “Osteoblast function on synthetic biodegradable polymers,” J. Biomed. Mater. Res. 1994, 28, 14451453.
2.) Ishaug-Riley, S. L.; Crane, G. M.; Gurlek, A.; Miller, M. J.; Yasko, A.; Yaszemski, M. J.; Mikos, A. G., “Ectopic bone formation by marrow stromal osteoblast transplantation using poly(D,Llactic-co-glycolic acid) foams implanted into the rat mesentery,” J. Biomed. Mater. Res. 1997, 36, 18.
3.) Ishaug, S. L.; Crane, G. M.; Miller, M. J.; Yasko, A.; Yaszemski, M. J.; Mikos, A. G., “Bone formation by three-dimensional stromal osteoblast culture in biodegradable polymer scaffolds,” J. Biomed. Mater. Res. 1997, 36, 1728.
4.) Attawia, M. A.; Herbert, K. M.; Laurencin, C. T., “Osteoblast-like cell adherance and migration through 3-dimensional porous polymer matrices,” Biochem. Biophys. Res. Commun. 1995, 213(2), 639644.
5.) DeLeu, J.; Trueta, J., “Vasularization of bone grafts in the anterior chamber of the eye,” J. Bone Joint Surg. 1965, 47B, 319.
6.) Heslop, B. F.; Zeiss, I. M.; Nisbet, N. W., “Studies on transference of bone: I. Comparison of autologous and homologous implants with reference to osteocyte survival, osteogenesis, and host reaction,” Br. J. Exp. Pathol. 1960, 41, 269.
7.) Yaszemski, M. J.; Payne, R. G.; Hayes, W. C.; Langer, R.; Mikos, A. G., “In vitro degradation of a poly(propylene fumarate)-based composite material,” Biomater. 1996, 17(22), 21272130.
8.) Szem, J. W.; Marra, K. G.; Kumta, P. N.; Cook, L. A.; DiMilla, P. A.; Weiss, L. E.Osteoblast adhesion and proliferation on polymer blends,” J. Biomed. Mater. Res., In Preparation.
9.) U.S. Patent, 1996, Mikos, A. G.; Sarakinos, G.; Vacanti, J. P.; Langer, R. S.; Cima, L. G., "Biocompatible polymer membranes and methods of preparation of three dimensional membrane structures,” 5,514,378.
10.) Marra, K.G.; Szem, J.W.; Kumta, P.N.; DiMilla, P.A.; Weiss, L.E.; “In Vitro Analysis of Biodegradable Polymer Blend/Hydroxyapatite Composites for Bone Tissue Engineering,” J. Biomed. Mat. Res. Submitted 7/98.

Novel three Dimensional Biodegradable Scaffolds for Bone Tissue Engineering

  • Kacey G. Marra (a1), Phil G. Campbell (a1), Paul A. Dimilla (a2), Prashant N. Kumta (a1) (a3), Mark P. Mooney (a4), Jeffrey W. Szem (a5) and Lee E. Weiss (a1) (a6)...

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed