Skip to main content Accessibility help
×
Home

Nano-fibrous scaffolding architecture enhances protein adsorption and cell attachment

  • Kyung Mi Woo (a1), Victor J. Chen (a1) and Peter X. Ma (a1)

Abstract

Tissue engineering aims at resolving problems such as donor shortage and immune rejection faced in transplantation. Scaffolds (artificial extracellular matrices) play critical roles in tissue engineering. Recently, we developed nano-fibrous poly(L-lactic acid) (PLLA) scaffolds under the hypothesis that synthetic nano-fibrous scaffolding, mimicking the structure of natural collagen fibers, could create a more favorable microenvironment for cells. This is the first report that the nano-fibrous architecture built in three-dimensional scaffolds improved the features of protein adsorption, which mediates cell interactions with scaffolds. Scaffolds with nano-fibrous pore walls adsorbed 4 times more serum proteins than scaffolds with solid pore walls. More interestingly, the nano-fibrous architecture selectively enhanced protein adsorption including fibronectin and vitronectin, even though both scaffolds were made from the same PLLA material. Furthermore, nano-fibrous scaffolds also allowed more than 1.7 times of osteoblastic cell attachment than scaffolds with “solid” pore walls. These results demonstrate that the biomimetic nano-fibrous architecture serves as superior scaffolding for tissue engineering.

Copyright

References

Hide All
1. Elsdale, T., Bard, J.. J. Cell Biol. 54, 626637 (1972).
2. Strom, S.C., Michalopoulos, G.. MethodsEnzymol. 82, 544555 (1982).
3. Grinnell, F., Bennett, M.H.. Methods Enzymol. 82, 535544 (1982).
4. Ma, P.X., Zhang, R.. J. Biomed. Mater. Res. 46, 6072 (1999).
5. Zhang, R., Ma, P.X.. J. Biomed. Mater. Res. 52, 430438 (2000).

Nano-fibrous scaffolding architecture enhances protein adsorption and cell attachment

  • Kyung Mi Woo (a1), Victor J. Chen (a1) and Peter X. Ma (a1)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed