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Microfluidics and Beyond – Devices for Applications in Biotechnology -

Published online by Cambridge University Press:  15 March 2011

Martina Daub ,
Rolf M. Kaack ,
Oliver Gutmann ,
Chris P. Steinert ,
Remigius Niekrawietz ,
Peter Koltay ,
Bas de Heij  and
Roland Zengerle
Martina Daub
Affiliation:
IMTEK - Institute for Microsystem Technology, University of Freiburg, Laboratory for MEMS Applications, Georges-Koehler-Allee 106, D-79110 Freiburg, Germany
Rolf M. Kaack
Affiliation:
IMTEK - Institute for Microsystem Technology, University of Freiburg, Laboratory for MEMS Applications, Georges-Koehler-Allee 106, D-79110 Freiburg, Germany
Oliver Gutmann
Affiliation:
IMTEK - Institute for Microsystem Technology, University of Freiburg, Laboratory for MEMS Applications, Georges-Koehler-Allee 106, D-79110 Freiburg, Germany
Chris P. Steinert
Affiliation:
IMTEK - Institute for Microsystem Technology, University of Freiburg, Laboratory for MEMS Applications, Georges-Koehler-Allee 106, D-79110 Freiburg, Germany
Remigius Niekrawietz
Affiliation:
IMTEK - Institute for Microsystem Technology, University of Freiburg, Laboratory for MEMS Applications, Georges-Koehler-Allee 106, D-79110 Freiburg, Germany
Peter Koltay
Affiliation:
IMTEK - Institute for Microsystem Technology, University of Freiburg, Laboratory for MEMS Applications, Georges-Koehler-Allee 106, D-79110 Freiburg, Germany
Bas de Heij
Affiliation:
IMTEK - Institute for Microsystem Technology, University of Freiburg, Laboratory for MEMS Applications, Georges-Koehler-Allee 106, D-79110 Freiburg, Germany
Roland Zengerle
Affiliation:
IMTEK - Institute for Microsystem Technology, University of Freiburg, Laboratory for MEMS Applications, Georges-Koehler-Allee 106, D-79110 Freiburg, Germany
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Abstract

For the performance of certain analytical and diagnostic tasks in modern Life Science applications high throughput screening (HTS) methods are essential. Miniaturization, parallelization and automation allow to decrease consumption of expensive materials and lead to faster analyzing times. The miniaturization of total assay volumes by the use of microtiter plates as well as the microarray technology have revolutionized the field of biotechnology and Life Sciences. Neither printing of microarrays with droplet volumes of several picoliters, nor handling of precious enzymes in the upper nanoliter range can be accomplished with traditional liquid handling devices like air displacement pipettes. The development of novel low volume liquid handling devices, which are subject to current research, addresses the diverse requirements shifting steadily to lower volumes. Various novel non-contact dispensing methods in the nanoliter and picoliter range are presented and classified according to their working principles like air displacement and direct displacement methods (TopSpot®, NanoJetTM, Dispensing Well PlateTM). Properties of the various methods are compared in terms of flexibility, integration density, speed of operation, precision, addressable volume range and amenability to multi-parallel operation.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 820: Symposia O/R – Nanoengineered Assemblies and Advanced Micro/Nanosystems , 2004 , R6.6
Copyright
Copyright © Materials Research Society 2004

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References

1. Ducrée, J., Zengerle, R.; “Flow Map – Microfluidics Roadmap for the Life Sciences”; edited by Ducrée, J. and Zengerle, R., ISBN code: 3-8334-0744-1, 2004, Books on Demand, Norderstedt, Germany (also available at www.microfluidics-roadmap.com).Google Scholar
2. Koltay, P., Ducrée, J., Zengerle, R.; “Nanoliter and Picoliter Liquid Handling”; edited by Oosterbroek, Edwin and Berg, Albert van den; Elsevier; Elsevier Science, Amsterdam; ISBN code: 0- 444-51100-8; September 2003; pp. 151–171.Google Scholar
3. Heij, B. de, Daub, M., Gutmann, O., Niekrawietz, R., Sandmaier, H., Zengerle, R.; “TopSpot - Highly Parallel Dispensing of Chemical and Biological Reagents”; Analytical and Bioanalytical Chemistry; Vol. 378, 2004; pp. 119–122.Google Scholar
4. Steinert, C.P., Goutier, I., Heij, B. de, Gutmann, O., Daub, M., Sandmaier, H., Messner, S., Zengerle, R.; “An improved 24 channel picoliter dispenser based on direct liquid displacement”; Proc. of IEEE-Transducers 2003, 8-12.06.2003, Boston, USA, 2003, pp. 376–379.Google Scholar
5. Gutmann, O., Kuehlewein, R., Reinbold, S., Niekrawietz, R., Steinert, C.P., Heij, B. de, Zengerle, R., Daub, M.; “A highly parallel nanoliter dispenser for microarray fabrication”; in press Biomedical Microdevices 6:2. 2004, pp. 131–137.Google Scholar
6. Steger, R., Koltay, P., Birkle, G., Strobelt, T., Sandmaier, H., Zengerle, R.; “A Two-Dimensional Array of Piezostack Actuated Nanoliter Dispensers”; Actuator 2002; 10-12 June 2002; Bremen; Germany, pp. 537–541.Google Scholar
7. Koltay, P., Steger, R., Birkle, G., Huang, H.-C., Sandmaier, H., Zengerle, R.; “Microdispenser array for highly parallel and accurate liquid handling”; SPIE's International Symposium on Microelectronics and Micro-Electro-Mechanical Systems; 17-19. December 2001, Adelaide, Australia.Google Scholar
8. Lindemann, T., Streule, W., Birkle, G., Zengerle, R., Koltay, P.; “PipeJet - A simple disposable dispenser for the nanoliter range”; to be published, Proc. Actuator 2004; Bremen; Germany; 14-16 June, 2004.Google Scholar
9. Koltay, P., Birkenmeier, B., Steger, R., Sandmaier, H., Zengerle, R.; “Massive Parallel Liquid Dispensing in the Nanoliter Range by Pneumatic Actuation”; Actuator 2002; 8th International Conference on New Actuators, 10-12 June 2002; Bremen; Germany, pp. 235–239.Google Scholar
10. Koltay, P., Bohl, B., Taoufik, S., Steger, R., Messner, S., Sandmaier, H., Zengerle, R.; “Dispensing Well Plate (DWP): A highly integrated nanoliter dispensing system”; Proc. of IEEE- Transducers 2003; 8-12.06.2003; Boston; USA; pp. 16–19.Google Scholar
11. Kaack, R.M., Jung, A., Wenz, H.M., Zengerle, R., Daub, M.; “PCR-slide: A modular and cascadable platform for DNA sample processing with integrated nanolitre dosage”; Proc. IEEE MEMS 2004, pp. 338–342.Google Scholar