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Loss of Infrared Linear Dichroism in Collagen Fibers as a Measure of Deterioration in Skin and Semi-Tanned Leather Artifacts

Published online by Cambridge University Press:  28 February 2011

Gregory S. Young
Gregory S. Young*
Affiliation:
Canadian Conservation Institute, Department of Communications, Ottawa, Canada K1A-0C8
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Abstract

Polarized radiation is used in a Fourier transform infrared (FTIR) microspectroscopic technique to detect molecular conformational change in collagen fibers resulting from deterioration. Absorbance spectra of single fibers are produced with polarization oriented first parallel (A‖) and then perpendicular (A⊥) to the fiber axis. The amide Ill band (1230- 1300 cm−1) shows strong positive dichroism, with up to six times greater absorbance occurring with parallel polarization. Losses of dichroism result from breakdown in the molecular structure of collagen. Integrations of the amide III bands are used in ratios, A‖/A⊥;, which provide a measure of the degree of molecular order. Ratios for degraded and undegraded material are compared to produce semi-quantitative information about deterioration.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 267: Symposium J – Materials Issues in Art and Archaeology III , 1992 , 859
Copyright
Copyright © Materials Research Society 1992

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