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Fast Detection of Perkinsus Marinus, a Prevalent Pathogen of Oysters and Clams from Sea Waters

  • Yu-Lin Wang (a1), B.H. Chu (a2), K.H. Chen (a3), Chih-Yang Chang (a4), Tanmay P. Lele (a5), George Papadi (a6), James K. Coleman (a7), Barbara J. Sheppard (a8), Chris F. Dungan (a9), S. J. Pearton (a10), J.W. Johnson (a11) and F. Ren (a12)...

Abstract

Antibody-functionalized, Au-gated AlGaN/GaN high electron mobility transistors (HEMTs) were used to detect Perkinsus marinus. The antibody was anchored to the gate area through immobilized thioglycolic acid. The AlGaN/GaN HEMT were grown by a molecular beam epitaxy system (MBE) on sapphire substrates. Infected sea waters were taken from the tanks in which Tridacna crocea infected with P. marinus were living and dead. The AlGaN/GaN HEMT showed a rapid response of drain-source current in less than 5 seconds when the infected sea waters were added to the antibody-immobilized surface. The recyclability of the sensors with wash buffers between measurements was also explored. These results clearly demonstrate the promise of field-deployable electronic biological sensors based on AlGaN/GaN HEMTs for Perkinsus marinus detection.

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Keywords

Fast Detection of Perkinsus Marinus, a Prevalent Pathogen of Oysters and Clams from Sea Waters

  • Yu-Lin Wang (a1), B.H. Chu (a2), K.H. Chen (a3), Chih-Yang Chang (a4), Tanmay P. Lele (a5), George Papadi (a6), James K. Coleman (a7), Barbara J. Sheppard (a8), Chris F. Dungan (a9), S. J. Pearton (a10), J.W. Johnson (a11) and F. Ren (a12)...

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