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Enzyme-Pretreatment Removes In Vitro Cytoxic Effects of Dermal Sheep Collagen

Published online by Cambridge University Press:  15 February 2011

M. J. A. van Luyn ,
P. B. van Wachem ,
L. H. H. Olde Damink ,
P. J. Dijkstra ,
J. Feijen  and
P. Nieuwenhuis
M. J. A. van Luyn
Affiliation:
Department of Histology and Cell Biology, Section Biomaterials Research, University of Groningen, Oostersingel 69/2, 9713 EZ Groningen, The Netherlands.
P. B. van Wachem
Affiliation:
Department of Histology and Cell Biology, Section Biomaterials Research, University of Groningen, Oostersingel 69/2, 9713 EZ Groningen, The Netherlands.
L. H. H. Olde Damink
Affiliation:
Department of Chemical Technology, Twente University, p.o. Box 217, 7500 AE Enschede, The Netherlands.
P. J. Dijkstra
Affiliation:
Department of Chemical Technology, Twente University, p.o. Box 217, 7500 AE Enschede, The Netherlands.
J. Feijen
Affiliation:
Department of Chemical Technology, Twente University, p.o. Box 217, 7500 AE Enschede, The Netherlands.
P. Nieuwenhuis
Affiliation:
Department of Histology and Cell Biology, Section Biomaterials Research, University of Groningen, Oostersingel 69/2, 9713 EZ Groningen, The Netherlands.
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Abstract

We investigated the in vitro cytotoxicity of commercially available hexamethylenediisocyanate-crosslinked dermal sheep collagen (HDSC). HDSC was found to induce medium cytotoxic effects, as measured with methylcellulose cell culture. Apart from primary cytotoxicity, due to direct release of (extractable) cytotoxic products, HDSC was found to contain secondary cytotoxicity, possibly released by enzymatic interactions. In this study we found proof for this hypothesis, by exposing extracted HDSC to enzyme-containing medium. Furthermore we observed, that enzymatic pre-treatment can remove all secondary cytotoxic products, possibly due to detachment of pendants, which are still coupled to fragments of collagen molecules. The possibility of enzymatic pretreatment of HDSC, to obtain a non-cytotoxic/biocompatible material, may be important for in vivo applications.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 252: Symposium T – Tissue-Inducing Biomaterials , 1991 , 167
Copyright
Copyright © Materials Research Society 1992

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References

REFERENCES

1. Chvaphil, M. Speer, D., Mora, W., Eskelson, C., Effect of tanning agent on tissue reaction to tissue implanted collagen sponge, J. Surg. Res. 35, 402–409, 1983.Google Scholar
2. Van Wachem, P.B., Van Luyn, M.J.A., Koerten, H.K., Olde Damink, L., Ten Hoopen, H., Feijen, J., Nieuwenhuis, P., In vivo degradation of processed dermal sheep collagen evaluated with transmission electron microscopy, Biomaterials, 12, 215–223, 1991.CrossRefGoogle ScholarPubMed
3. Van Wachem, P.B., Van Luyn, M.J.A., Olde Damink, L., Feijen, J., Nieuwenhuis, P., Tissue interactions with dermal sheep collagen implants: a transmission electron microscopical evaluation, Cells and Materials, 1, 3, 251–263, 1991.Google Scholar
4. Ulreich, J.B., Chvapil, M., A quantitative microassay for in vitro toxicity testing of biomaterials, J. Biomed. Mater. Res., 15, 913–922, 1981.Google Scholar
5. Van Luyn, M.J.A., Van Wachem, P.B., Olde Damink, L., Ten Hoopen, H., Feijen, J., Nieuwenhuis, P., Methylcellulose culture as a new cytotoxicity test system for biomaterials, J. of Mat. Sci.: Mater. in Med., 2, 142–148, 1991.Google Scholar
6. Van Luyn, M.J.A., Van Wachem, P.B., Olde Damink, L.H.H., Dijkstra, P. J., Feijen, J., Nieuwenhuis, P., Relations between in vitro cytotoxicity and crosslinked dermal sheep collagens, Submitted to J. Biomed. Mater. Res.Google Scholar
7. Van Luyn, M.J.A., Van, Wachem, Jonkman, M.H., , J., Nieuwenhuis, P., Cytotoxicity testing of wound dressings using methylcellulose cell culture, accepted for publication in Biomaterials, 1991 CrossRefGoogle Scholar
8. Iscove, N.N. and Schreier, H., In: Clonal Growth of Cells in Semisolid or Viscous Medium, Immunological Methods, Academic Press, 1979.Google Scholar
9. Berode, M., Leuenberger, Ph. and Savolainen, H., Phenotype-dependent Inhibition of Human αl-antitrypsin by 1,6-Hexanediamine in Vitro, Biochem. Int.. 17, 1, 1988.Google Scholar
10. Yano, E., Yoshioka, M. and Koizumi, A., Relationship between chemical structure and cytotoxicity of aliphatic amines examined by a microtiter system with cultured fibroblasts, Jpn. J. Ind. Health, 23, 537–543, 1981.Google Scholar
11. Johannsen, F.R. and Levinskas, G.J., Toxicological Profile of orally administered 1,6-Hexanediamine in the rat, J. of Applied Toxicol. 7, 4, 259–263, 1987.Google Scholar
12.. Murphy, G., Reynolds, J.J., Current views of collagen degradation, Progress towards understanding the resorption of connective tissues, Bio Essays 2, 55–60, 1985.Google Scholar
13. Yajima, T., Acid phosphatase activity and intracellular collagen degradation by fibroblasts in vitro, Cell and Tissue Research, 245, 253–260, 1986.Google Scholar
14.14. Woolley, D.E., Mammalian collagenases, In: Extracellular Matrix Biochemistry, Eds. Piez, K.A. and Reddi, A.H., Elsevier, New York, 119–157, 1984.Google Scholar
15. Lazarus, S.L., and Fullmer, H.M., Collagenase production by human dermis in vitro, J. of Invest. Derm., 52, 6, 1969.Google Scholar
1. Harris, E D., Krane, S. M., Collagenases: Specific involement of collagenase in normal physiology and pathology, The New. Eng. J. of Med. 652–661, 1974 CrossRefGoogle Scholar
17. Kleiman, H. K., Klebe, R. J., and Martin, G.R. Role of Collagenous Matrices in the Adhesion and Growth of Cells, J. Cell Biol., 88, 473–485, 1981.CrossRefGoogle Scholar
18. Nimni, M.E., and Harkness, R., D., Molecular Structure of Collagen, In: Collagen, Vol.1, Chapter 1, Ed. Nimni, M.E., CRC Press, Inc., Boca Raton Florida, U.S.A., 1988.Google Scholar
19. Fields, G.B., Van Wart, H. E., and Birkedal-Hansen, H., Sequence specificity of human skin fibroblast collagenase, The J. of Biol. Chem., Vol.262, 6221–6226,1987.Google Scholar