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Enzymatic Biosensors with Integrated Thin Film a-Si:H Photodiodes

  • Ana Teresa Pereira (a1), Virginia Chu (a2), Duarte M. F. Prazeres (a3) and Joao P. Conde (a4)

Abstract

A microfabricated amorphous silicon photodiode is used to detect chemiluminescent and colorimetric horseradish peroxidase (HRP) enzymatic reactions. Detections limits of 1 nM and 1 pM of HRP are obtained for chemiluminescent and colorimetric measurements, respectively, with the reactions carried out in solution volume of 50 μL in polystyrene microwells. Surface-adsorbed HRP can be detected with a limit of 1 fmol.cm-2 by both detection methods. Immunoassays were performed using HRP-labeled antibodies and the detection of specific antibody-antigen molecular recognition is demonstrated both in the plastic well and inside a microfluidic channel. The application of the a-Si:H/HRP system is extended by coupling HRP with oxidase enzyme systems for glucose detection and a sensitivity of 0.1 mmol/L was achieved.

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[1] Azevedo, A. M. et al. , “Horseradish peroxidase: a valuable tool in biotechnology,” Biotechnology Annual Review, Vol 9, vol. 9, pp. 199247, 2003.
[2] Street, R. A., Hydrogenated amorphous silicon: Cambridge University Press, 1991.
[3] Pimentel, A. C. et al. , “Detection of chemiluminescence using an amorphous silicon photodiode,” Ieee Sensors Journal, vol. 7, pp. 415416, Mar-Apr 2007.
[4] Gouvea, A. et al. , “Colorimetric detection of molecular recognition reactions with an enzyme biolabel using a thin-film amorphous silicon photodiode on a glass substrate,” Sensors and Actuators B-Chemical, vol. 135, pp. 102107, Dec 2008.
[5] Pereira, A. T. et al. , “Chemiluminescent Detection of Horseradish Peroxidase Using an Integrated Amorphous Silicon Thin Film Photosensor,” IEEE Sensors Journal, Accepted for publication.

Keywords

Enzymatic Biosensors with Integrated Thin Film a-Si:H Photodiodes

  • Ana Teresa Pereira (a1), Virginia Chu (a2), Duarte M. F. Prazeres (a3) and Joao P. Conde (a4)

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