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Electrical Detection of Protein Biomarkers Using Nanoneedle Biosensors

Published online by Cambridge University Press:  14 May 2012

Rahim Esfandyarpour ,
Hesaam Esfandyarpour ,
Mehdi Javanmard ,
James S. Harris  and
Ronald W. Davis
Rahim Esfandyarpour
Affiliation:
Center for Integrated Systems, Department of Electrical Engineering, Stanford University Stanford Genome Technology Center; 855 California Ave., Palo Alto, CA 94304, USA Phone: +1-650-387-5976, Email: rahimes@stanford.edu
Hesaam Esfandyarpour
Affiliation:
Center for Integrated Systems, Department of Electrical Engineering, Stanford University
Mehdi Javanmard
Affiliation:
Stanford Genome Technology Center; 855 California Ave., Palo Alto, CA 94304, USA Phone: +1-650-387-5976, Email: rahimes@stanford.edu
James S. Harris
Affiliation:
Center for Integrated Systems, Department of Electrical Engineering, Stanford University
Ronald W. Davis
Affiliation:
Stanford Genome Technology Center; 855 California Ave., Palo Alto, CA 94304, USA Phone: +1-650-387-5976, Email: rahimes@stanford.edu
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Abstract:

Here we present the development of an array of electrical nano-biosensors in a microfluidic channel, called Nanoneedle biosensors. Then we present the proof of concept study for protein detection. A Nanoneedle biosensor is a real-time, label-free, direct electrical detection platform, which is capable of high sensitivity detection, measuring the change in ionic current and impedance modulation, due to the presence or reaction of biomolecules such as proteins or nucleic acids. We show that the sensors which have been fabricated and characterized for the protein detection. We have functionalized Nanoneedle biosensors with receptors specific to a target protein using physical adsorption for immobilization. We have used biotinylated bovine serum albumin as the receptor and sterptavidin as the target analyte. The detection of streptavidin binding to the receptor protein is also presented.

Keywords

sensorbiomedicalelectrical properties
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1414: Symposium HH – Bioelectronics–Materials, Properties and Applications , 2012 , mrsf11-1414-hh04-04
Copyright
Copyright © Materials Research Society 2012

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References

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