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Effects of Hydration on Nanoscale Structural Morphology and Mechanics of Individual Type I Collagen Fibrils

Published online by Cambridge University Press:  18 May 2012

Joseph M. Wallace ,
Chad Harding  and
Arika Kemp
Joseph M. Wallace
Affiliation:
Department of Biomedical Engineering, Indiana University-Purdue University at Indianapolis, 723 W Michigan St. SL220, Indianapolis, IN 46202, U.S.A.
Chad Harding
Affiliation:
Department of Biomedical Engineering, Indiana University-Purdue University at Indianapolis, 723 W Michigan St. SL220, Indianapolis, IN 46202, U.S.A.
Arika Kemp
Affiliation:
Department of Biomedical Engineering, Indiana University-Purdue University at Indianapolis, 723 W Michigan St. SL220, Indianapolis, IN 46202, U.S.A.
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Abstract

Type I collagen is one of the most vital proteins in our bodies and serves a number of structural roles. Despite collagen’s importance, little is known about its nanoscale morphology in tissues and how morphology relates to mechanical function. This study directly probes nanoscale structure and mechanics in collagen as a function of hydration utilizing atomic force microscopy investigations of the mouse tail tendon. We demonstrate that collagen morphology and mechanical properties at the nanoscale change with dehydration, indicating that hydration is a factor which must be considered when performing studies at any length scale in collagen-based tissues. Studies are underway to further investigate this phenomenon and to determine how these properties change with disease in tendon and other Type I collagen-based tissues.

Keywords

scanning probe microscopy (SPM)nanostructurenano-indentation
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1465: Symposium SS – Structure/Property Relationships in Biological and Biomimetic Materials at the Micro-, Nano- and Atomic-Length Scales , 2012 , mrss12-1465-ss02-02
Copyright
Copyright © Materials Research Society 2012

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References

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