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Covalent Attachment of a Single Dextran Polymer Between the Tip of an Atomic Force Microscope and a Gold Surface

Published online by Cambridge University Press:  10 February 2011

M. Grandbois ,
R. Décor ,
M. Rief ,
A. Wagner ,
C. Mioskowski  and
Hermann Gaub
M. Grandbois
Affiliation:
Lehrstuhl für Angewandte Physik, Ludwig-Maximilians-Universität, Amalienstrasse 54, D-80799, München, Germany
R. Décor
Affiliation:
Laboratoire de synthèse bioorganique, UMR 7514 CNRS Université Louis Pasteur de Strasbourg, Faculté de pharmacie, 74 route du Rhin, BP 24, 67401 Illkirch, France
M. Rief
Affiliation:
Lehrstuhl für Angewandte Physik, Ludwig-Maximilians-Universität, Amalienstrasse 54, D-80799, München, Germany
A. Wagner
Affiliation:
Laboratoire de synthèse bioorganique, UMR 7514 CNRS Université Louis Pasteur de Strasbourg, Faculté de pharmacie, 74 route du Rhin, BP 24, 67401 Illkirch, France
C. Mioskowski
Affiliation:
Laboratoire de synthèse bioorganique, UMR 7514 CNRS Université Louis Pasteur de Strasbourg, Faculté de pharmacie, 74 route du Rhin, BP 24, 67401 Illkirch, France
Hermann Gaub
Affiliation:
Lehrstuhl für Angewandte Physik, Ludwig-Maximilians-Universität, Amalienstrasse 54, D-80799, München, Germany
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Abstract

A method for covalent immobilization of a single dextran polymer between a gold surface and the tip of an atomic force microscope (AFM) is presented. Carboxymethylated dextran immobilized on gold by epoxythiol chemistry was activated with N-hydroxysuccinimide (NHS) and N-ethyl-N'-(dimethylaminopropyl) carbodiimide hydrochloride (EDC) in order to make the dextran polymer reactive for the amino groups present on the previously aminosilanized AFM tip. By measuring force vs extension curves we have shown that it is possible to catch such an activated dextran polymer with an AFM tip through the formation of a covalent bond. Dextran polymers were attached even without any detectable indentation of the tip in the dextran-coated gold surface. In this so-called fly-fishing mode, attachment of multiple dextran polymers, which typically occurs when the tip is indented into the surface, are efficiently avoided.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 576: Symposium DD – Organic/Inorganic Hybrid Materials II , 1999 , 171
Copyright
Copyright © Materials Research Society 1999

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References

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