Hostname: page-component-76fb5796d-wq484 Total loading time: 0 Render date: 2024-04-26T22:45:52.934Z Has data issue: false hasContentIssue false
  • English
  • Français

Characterization of Erythrocytes in the Sickle Cell Trait

Published online by Cambridge University Press:  21 March 2011

Jamie L. Maciaszek  and
George Lykotrafitis
Jamie L. Maciaszek
Affiliation:
Department of Mechanical Engineering, University of Connecticut, Storrs, CT 06269, U.S.A.
George Lykotrafitis
Affiliation:
Department of Mechanical Engineering, University of Connecticut, Storrs, CT 06269, U.S.A.
Get access

Abstract

Atomic force microscopy (AFM) allows for high-resolution topography studies of biological cells, measurement of their mechanical properties, and quantification of protein-protein interactions in physiological conditions. In this work, AFM was employed to investigate morphological, material, and chemomechanical properties of red blood cells from human subjects with sickle cell trait. We measured the stiffness of the cells and demonstrated that the Young’s modulus of pathological erythrocytes was three times greater than in normal cells. A single molecule AFM method was employed to report that erythrocytes from human subjects with the sickle cell trait express a greater number of the laminin receptors BCAM/Lu (p < 0.05) than erythrocytes from normal human subjects. Observed differences indicate the effect of sickle hemoglobin in the erythrocyte and possible changes in the organization of the cell cytoskeleton and membrane proteins associated with the sickle cell trait.

Keywords

adhesionelastic propertiesnano-indentation
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1301: Symposium V/NN/OO/PP – Soft Matter, Biological Materials and Biomedical Materials—Synthesis, Characterization and Applications , 2011 , mrsf10-1301-oo16-08
Copyright
Copyright © Materials Research Society 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

[1] Ferrone, F. A., “Polymerization and sickle cell disease: A molecular view,” Microcirculation, vol. 11, pp. 115–128, Mar-Apr 2004.Google Scholar
[2] Wells, I. C. and Itano, H. A., “Ratio of sickle-cell anemia hemoglobin to normal hemoglobin in sicklemics,” J Biol Chem, vol. 188, pp. 65-74, 1951 1951.Google Scholar
[3] Tsaras, G., et al. , “Complications Associated with Sickle Cell Trait: A Brief Narrative Review,” The American Journal of Medicine, vol. 122, pp. 507–512, 2009.Google Scholar
[4] Walder, J. A., et al. , “The Interaction of Hemoglobin with the Cytoplasmic Domain of Band-3 of the Human-Erythrocyte Membrane,” Journal of Biological Chemistry, vol. 259, pp. 238–246, 1984.Google Scholar
[5] An, X. L., et al. , “Adhesive activity of Lu glycoproteins is regulated by interaction with spectrin,” Blood, vol. 112, pp. 5212–5218, 2008.Google Scholar
[6] Sneddon, I., “The relation between load and penetration in axisymmetric Boussinesq problem for a punch of arbitrary profile,” Int J Eng Sci, vol. 3, pp. 47–57, 1965.Google Scholar
[7] Hategan, A., et al. , “Adhesively-tensed cell membranes: Lysis kinetics and atomic force microscopy probing,” Biophysical Journal, vol. 85, pp. 2746–2759, 2003.Google Scholar
[8] Bilodeau, G. G., “Regular Pyramid Punch Problem,” Journal of Applied Mechanics, vol. 59, pp. 519–523, 1992.Google Scholar
[9] Liu, F., et al. , “Calcium-dependent human erythrocyte cytoskeleton stability analysis through atomic force microscopy,” Journal of Structural Biology, vol. 150, pp. 200–210, 2005.Google Scholar