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Cell Interactions With Fibronectin-Coated Electrically Conducting Polypyrrole Thin Films

Published online by Cambridge University Press:  15 February 2011

Joyce Y. Wong ,
Robert Langer  and
Donald E. Ingber
Joyce Y. Wong
Affiliation:
Massachusetts Institute of Technology, Cambridge, MA 02139
Robert Langer
Affiliation:
Massachusetts Institute of Technology, Cambridge, MA 02139
Donald E. Ingber
Affiliation:
Depts. of Surgery and Pathology, Children's Hospital and Harvard Medical School, Boston, MA 02115
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Abstract

Surface properties of electrically conducting polymers, including charge-density and wettability, can be altered reversibly by oxidation or reduction. Thus, these materials are interesting systems on which to study the interactions of proteins and cells with surfaces. The properties of polypyrrole were studied in environments suitable for protein adsorption and cell culture. Optically transparent films of polypyrrole were synthesized, and the stability of the oxidized and reduced states in aqueous media were studied. UV/VIS spectroscopy, cyclic voltammetry, and spectroelectrochemistry measurements confirmed the oxidation state of the polymer films. The positively-charged form of polypyrrole could be changed to its neutral form by applying a constant potential (-0.5 V vs. Ag/AgCl reference). It was found that the shape of bovine aortic endothelial cells on fibronectin-coated polypyrrole films can be controlled by varying the oxidation state of the polypyrrole.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 331: Symposium T – Biomaterials for Drug and Cell Delivery , 1993 , 141
Copyright
Copyright © Materials Research Society 1994

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