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Biomimetic Surfaces via Dextran Immobilization: Grafting Density and Surface Properties

Published online by Cambridge University Press:  15 March 2011

Davide Miksa ,
Elizabeth R. Irish ,
Dwayne Chen ,
Russell J. Composto  and
David M. Eckmann
Davide Miksa
Affiliation:
Department of Anesthesia, University of Pennsylvania, Philadelphia, PA 19104
Elizabeth R. Irish
Affiliation:
Department of Material Science and Engineering, University of Pennsylvania, Philadelphia, PA 19104
Dwayne Chen
Affiliation:
Department of Anesthesia, University of Pennsylvania, Philadelphia, PA 19104
Russell J. Composto
Affiliation:
Department of Material Science and Engineering, University of Pennsylvania, Philadelphia, PA 19104
David M. Eckmann
Affiliation:
Department of Anesthesia, University of Pennsylvania, Philadelphia, PA 19104
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Abstract

Biomimetic surfaces were prepared by chemisorption of oxidized dextran (Mw = 110 kDa) onto SiO2 substrates that were previously modified with aminopropyl-tri-ethoxy silane (APTES). The kinetics of dextran oxidation by sodium metaperiodate (NaIO4) were quantified by 1H NMR and pH measurements. The extent of oxidation was then used to control the morphology of the biomimetic surface. Oxidation times of 0.5, 1, 2, 4, and 24 hours resulted in <20, ∼30, ∼40, ∼50 and 100% oxidation, respectively. The surfaces were characterized by contact angle analysis and atomic force microscopy (AFM). Surfaces prepared with low oxidation times revealed a more densely packed “brushy” layer when imaged by AFM than those prepared at low oxidation times. Finally, the contact angle data revealed, quite unexpectedly, that the surface with the greatest entropic freedom (0.5 h) wetted the fastest and to the greatest extent (θAPTES>θ1h>θ2,4h>θ0.5h).

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 826: Symposium V – Proteins as Materials , 2004 , V2.2
Copyright
Copyright © Materials Research Society 2004

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References

(1) Cryer, A. Biochemical Interactions at the Endothelium; Elsevier: New York, 1983; pp 5–30.Google Scholar
(2) Dai, L.; St John, H. A. W.; Bi, J.; Zientek, P.; Chatelier, R. C. Surf.Inter.Analy. 2000, 29, 46–55.Google Scholar
(3) Hartley, P. G.; McArthur, S. L.; McLean, K. M.; Griesser, H. J. Langmuir 2002, 18, 2483–2494.Google Scholar
(4) Mason, M.; Vercruysse, K. P.; Kirker, K. R.; Frisch, R.; Marecak, D. M.; Prestwich, G. D.; Pitt, W. G. Biomaterials 2000, 21, 31–36.Google Scholar
(5) Elender, G.; Kuhner, M.; Sackmann, E. Biosensors & Bioelectronics 1996, 11, 565–577.Google Scholar
(6) Ditsent, V. E.; Skorokhodov, I. I.; Terenteva, N. A.; Zolotarev, M. N.; Belyakova, Z. V.; Belikova, Z. V. Zhu.Fizi.Khim. 1976, 50, 1905–1906.Google Scholar
(7) Hughes, G.; Nevell, T. P. Tran.Farad.Soc. 1948, 44, 941–948.Google Scholar
(8) Andrade, J.D. Poly.Sci.Tech. 1986, 34, 29–40.Google Scholar
(9) Holly, F.J.; Refojo, M.F. J.Biomed.Mate.Rese. 1975, 9, 315–326.Google Scholar